PMID: 9165110Apr 25, 1997Paper

Cooperativity: a unified view

Biochimica Et Biophysica Acta
Luis Acerenza, Eduardo Mizraji

Abstract

Cooperativity, the departure from hyperbolic behaviour of the fractional saturation of a receptor at equilibrium (Y) for different values of ligand concentration (L), is an essential property of many physiological mechanisms and a first clue to the existence of conformational transitions and allosteric interactions. Here we investigate the properties of a simple and sensitive procedure to test and quantify cooperative behaviour. The measure of cooperativity involved is kappa = dK(L)/dL where K(L) = (1- Y) L/Y= [free sites]L/[occupied sites] is called the 'global dissociation quotient' Cooperative behaviour appears when kappa is not equal to 0, i.e., K(L) is a function of L. We have shown, for several equilibrium models of cooperative behaviour (e.g., Monod-Wyman-Changeux and Koshland-Némethy-Filmer), that K(L) can be expressed as the weighted average of the microscopic dissociation constants (K(i)) where the weights are the corresponding fractions of occupied sites (X(i)), K(L)= sigmaK(i)X(i). As a consequence, the change in the global dissociation quotient with ligand concentration for a dimer is kappa = (K1 - K2)dX1/dL. This result shows that the quantitative importance of a cooperative behaviour in a dimer depends on two fac...Continue Reading

References

Nov 21, 1987·Journal of Theoretical Biology·E MizrajiJ Lin
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Wyman, P E Phillipson

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Citations

Apr 1, 2008·Journal of Biological Physics·Josep Lluís GarcésFrancesc Mas
Mar 21, 2006·Biochemistry. Biokhimii︠a︡·V N DavidovaT F Solov'eva
Feb 4, 2016·Chemical Reviews·A Subha Mahadevi, G Narahari Sastry
Mar 6, 2007·Journal of Inorganic Biochemistry·Małgorzata Wszelaka-RylikWojciech Bal
Jan 24, 2007·Journal of Theoretical Biology·Alexander Y MitrophanovMark Borodovsky
May 15, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexander Y Mitrophanov, Eduardo A Groisman
Nov 22, 2007·Biochemical and Biophysical Research Communications·Małgorzata RózgaWojciech Bal
Nov 14, 2012·Chemical Reviews·A Subha Mahadevi, G Narahari Sastry
Aug 14, 2013·Metallomics : Integrated Biometal Science·Anna StaszewskaWojciech Bal
Jul 16, 2011·Physical Chemistry Chemical Physics : PCCP·A Subha MahadeviG Narahari Sastry
Aug 22, 2001·Biochimica Et Biophysica Acta·E Luchter-Wasylewska

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