Cooperativity between Rad51 and C/EBP family transcription factors modulates basal and Tat-induced activation of the HIV-1 LTR in astrocytes

Journal of Cellular Physiology
Galina ChipitsynaS Amini

Abstract

Transcription of the HIV-1 genome is a complex event that requires functional and physical communication of cellular proteins that recognize the LTR sequence with viral proteins, most notably, Tat. Moreover, studies have revealed the ability of Tat to induce transcription of a variety of cellular genes whose products can affect the status of cells, thus contributing to the pathogenesis of AIDS. Recently, we demonstrated that expression of Tat in astrocytes and other neural cells leads to upregulation of Rad51, a major component of DNA repair via homologous recombination. The unscheduled upregulation of Rad51, in turn, has an impact upon the extent of chromosomal abnormalities that are seen in Tat-producing cells. Here, we asked whether an elevation in Rad51 levels influences the extent of viral gene transcription in astrocytic cells. Our results demonstrate that ectopic expression of Rad51 enhances the basal- and the Tat-induced transcription of the LTR promoter. This event requires cooperativity from the C/EBP family of transcription factors including C/EBPbeta and C/EBPbeta homologous protein (CHOP). Similar to Tat, we showed that Rad51 interacts with C/EBPbeta and augments its interaction with the DNA motif spanning nucleoti...Continue Reading

References

Jul 1, 1990·Genes, Chromosomes & Cancer·B VogelsteinJ Bos
Sep 16, 1988·Science·N Ahmad, S Venkatesan
Apr 24, 1996·Molecular & General Genetics : MGG·A YamamotoT Morita
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·R C GuptaC M Radding
Dec 31, 1997·Molecular and Cellular Biology·S J XiaR J Shmookler Reis
Jul 22, 1998·Journal of Neuroimmunology·B E SawayaS Amini
Mar 19, 1999·Molecular and Cellular Biology·Y HuangZ M Yuan
Apr 16, 1999·Journal of Leukocyte Biology·J RappaportK Khalili
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·G L GalliaK Khalili
Oct 19, 1999·Molecular and Cellular Biology·M UbedaJ F Habener
Jan 5, 2000·Molecular Cell·E Kowenz-Leutz, A Leutz
Jan 19, 2000·Nucleic Acids Research·L A PereiraN J Deacon
May 22, 2003·Virology·Selvajothi AbrahamShohreh Amini
Sep 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tobias N Cassel, Magnus Nord
Sep 10, 2003·Journal of Leukocyte Biology·Olivier RohrEvelyne Schaeffer
Oct 11, 2003·Molecular and Cellular Neurosciences·Hong PuMichal Toborek
Jan 20, 2004·Gene·Alessandro MarcelloMauro Giacca
Mar 31, 2004·Current HIV Research·Andrew P Rice, Christine H Herrmann
Apr 14, 2004·Current HIV Research·Kevin V Morris, John J Rossi
Aug 11, 2004·The Journal of Biological Chemistry·Shohreh AminiBassel E Sawaya
Aug 26, 2004·The Journal of Biological Chemistry·Jennifer L Lavrrar, Peggy J Farnham
Jan 27, 2005·Cancer Letters·Christine Richardson
Feb 16, 2005·Journal of Neuroimmunology·Selvajothi AbrahamShohreh Amini
Apr 16, 2005·Cell Death and Differentiation·M KaulS A Lipton

❮ Previous
Next ❯

Citations

May 23, 2014·PloS One·Rafal KaminskiKamel Khalili
Mar 31, 2009·Future Microbiology·Yujie LiuBrian Wigdahl
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wendy E DurrantXinnian Dong
May 17, 2012·Biochimica Et Biophysica Acta·Xiukun CuiMugen Liu
Jul 13, 2011·Biochimica Et Biophysica Acta·Slava RomFrancesca Peruzzi
Jun 9, 2015·Chemistry & Biology·Sylvain ThierryVincent Parissi
Oct 5, 2010·Cell Cycle·Inna RomKamel Khalili
Jun 2, 2016·Journal of Cellular Physiology·Satarupa SenPrasun K Datta
Dec 26, 2006·Journal of Cellular Physiology·Alessandra RosatiMaria Caterina Turco
Nov 23, 2017·Clinical Medicine Insights. Pathology·Yujie LiuBrian Wigdahl
May 9, 2014·Oncotarget·Adrian P WiegmansKum Kum Khanna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.