Cooperativity between two NF-kappa B complexes, mediated by high-mobility-group protein I(Y), is essential for cytokine-induced expression of the E-selectin promoter.

Molecular and Cellular Biology
H LewisJ Whelan

Abstract

Cytokine-induced expression of the E-selectin gene requires the promoter binding and interaction of the transcription factors NF-kappa B and ATF. Here we have further analyzed the E-selectin promoter and revealed an additional region (nucleotides -140 to -105 [-140/-105]) which is essential in controlling promoter activation by cytokines. We identified high-mobility-group protein I(Y) [HMG-I(Y)] interacting specifically at two sites within this region. We noted that one of the HMG-I(Y)-binding sites overlaps a sequence element (-127/-118) diverging at only one position from the NF-kappa B consensus binding sequence. This led us to ask whether the -127/-118 element represents a second functional NF-kappa B-binding site within the E-selectin promoter. Using specific antisera, we show that p50, p65, and, interestingly, RelB are components of the complex interacting at this site. Mutational analysis of the -127/-118 NF-kappa B site indicates that both NF-kappa B and HMG-I(Y) binding at this site are essential for interleukin-1 induction of the promoter. We demonstrate that the binding affinity of the p50 subunit of NF-kappa B to both NF-kappa B sites within the E-selectin promoter is significantly enhanced by HMG-I(Y). In addition,...Continue Reading

References

Apr 1, 1992·Trends in Biochemical Sciences·V BlankA Israël
Mar 20, 1992·Cell·J D ParvinP A Sharp
Feb 28, 1991·Nature·L J PickerE C Butcher
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K F MontgomeryJ M Harlan
Feb 1, 1992·Molecular and Cellular Biology·R P RyseckR Bravo
Aug 2, 1990·Nature·T A Springer
Sep 7, 1990·Cell·T D Gilmore
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J A MolitorW C Greene
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R B GaynorD S Sigman
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R ReevesK R Johnson
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·K FerreriM Montminy
Jun 1, 1993·Current Opinion in Cell Biology·H C Liou, D Baltimore
Jan 1, 1993·International Review of Cytology·M GrilliM J Lenardo

❮ Previous
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Citations

Jun 19, 2001·Biochimica Et Biophysica Acta·R Reeves, L Beckerbauer
Dec 29, 1998·Biochimie·S Lopez, S Navarro
Apr 12, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Curtis M TyreeElizabeth A Allegretto
May 11, 2002·Breast Cancer Research and Treatment·Christine E DoldeLinda M S Resar
Jun 11, 1999·The Plant Journal : for Cell and Molecular Biology·J F Martínez-García, P H Quail
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Takeuchi, V R Baichwal
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J F Maher, D Nathans
Jan 27, 2005·The Journal of Biological Chemistry·Leonard C EdelsteinTucker Collins
Aug 9, 2003·DNA and Cell Biology·Sandra Resto-RuizBurt E Anderson
Dec 2, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Raymond W GansterDavid A Geller
Jul 1, 1995·Molecular Biology of the Cell·M YebraD A Cheresh
Oct 15, 1996·Nucleic Acids Research·X ZhouK Chada
Jan 10, 2002·Critical Care Medicine·Irvith M CarvajalMark A Perrella
Apr 25, 2007·Molecular and Cellular Biology·Smarajit BandyopadhyayPaul E DiCorleto
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·N LeitingerD K Vora
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E GerritsenT Collins
Jun 26, 2007·Atherosclerosis·Chinmay M TrivediChandrashekhar V Patel
May 9, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H R BoumaT A Schuurs
Nov 20, 2014·Aging Cell·Elizabeth E PowterJennifer R Gamble
Apr 6, 2016·Frontiers in Genetics·Sebastian ZeidlerMehmet Gültas
Feb 24, 1995·Cell·D Thanos, T Maniatis

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