Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki

Proceedings of the National Academy of Sciences of the United States of America
Pavel V MazinOleg Gusev

Abstract

Polypedilum vanderplanki is a striking and unique example of an insect that can survive almost complete desiccation. Its genome and a set of dehydration-rehydration transcriptomes, together with the genome of Polypedilum nubifer (a congeneric desiccation-sensitive midge), were recently released. Here, using published and newly generated datasets reflecting detailed transcriptome changes during anhydrobiosis, as well as a developmental series, we show that the TCTAGAA DNA motif, which closely resembles the binding motif of the Drosophila melanogaster heat shock transcription activator (Hsf), is significantly enriched in the promoter regions of desiccation-induced genes in P. vanderplanki, such as genes encoding late embryogenesis abundant (LEA) proteins, thioredoxins, or trehalose metabolism-related genes, but not in P. nubifer Unlike P. nubifer, P. vanderplanki has double TCTAGAA sites upstream of the Hsf gene itself, which is probably responsible for the stronger activation of Hsf in P. vanderplanki during desiccation compared with P. nubifer To confirm the role of Hsf in desiccation-induced gene activation, we used the Pv11 cell line, derived from P. vanderplanki embryo. After preincubation with trehalose, Pv11 cells can ente...Continue Reading

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Citations

Aug 26, 2018·Proteomics·Brett JanisMichael A Menze
Jan 10, 2020·The Journal of Experimental Biology·Alice E Harada, Ronald S Burton
Jul 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alina RyabovaOleg Gusev
Jan 10, 2019·Frontiers in Physiology·Leena Thorat, Bimalendu B Nath
Jul 13, 2018·Genes·Briardo LlorenteHugh D Goold
Nov 17, 2020·Frontiers in Physiology·Jonathan D HibshmanBob Goldstein
Jun 3, 2021·International Journal of Molecular Sciences·Shoko TokumotoTakahiro Kikawada

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Datasets Mentioned

BETA
SRP070984
AB490338

Methods Mentioned

BETA
RNA-seq
MDS
transfection
PCR
Assay

Software Mentioned

TOMTOM
R
HOMER
BLAST
Primer3Plus
BLAT
SAJR
MASS package
orthoMCL
tophat

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