Coordinate expression of alpha-tropomyosin and caldesmon isoforms in association with phenotypic modulation of smooth muscle cells.

The Journal of Biological Chemistry
K KashiwadaK Sobue

Abstract

Isoform diversity of tropomyosin is generated from the limited genes by a combination of differential transcription and alternative splicing. In the case of the alpha-tropomyosin (alpha-TM) gene, exon 2a rather than exon 2b is specifically spliced in alpha-TM-SM mRNA, which is one of the major tropomyosin isoforms in smooth muscle cells. Here we demonstrate that expressions of alpha-tropomyosin and caldesmon isoforms are coordinately regulated in association with phenotypic modulation of smooth muscle cells. Molecular cloning and Western and Northern blottings have revealed that in addition to the down-regulation of beta-TM-SM, alpha-TM-SM converted to alpha-TM-F1 and alpha-TM-F2 by a selectional change from exon 2a to exon 2b during dedifferentiation of smooth muscle cells in culture. Simultaneously, a change of caldesmon isoforms from high Mr type to low Mr type was also observed by alternative selection between exons 3b and 4 in the caldesmon gene during this process. In contrast, cultured smooth muscle cells maintaining a differentiated phenotype continued to express alpha-TM-SM, beta-TM-SM, and high Mr caldesmon. In situ hybridization revealed specific coexpression of alpha-TM-SM and high Mr caldesmon in smooth muscle in d...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K HayashiK Sobue
Sep 1, 1992·Molecular and Cellular Biology·A GualbertoK Walsh
Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J P Lees-Miller, D M Helfman
Oct 7, 1991·FEBS Letters·A DraegerJ V Small
Nov 12, 1990·FEBS Letters·M GimonaJ V Small
Aug 15, 1989·European Journal of Biochemistry·A InoueT Masaki
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K SobueS Kakiuchi
Jan 1, 1986·Differentiation; Research in Biological Diversity·T VolbergB Geiger
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A GlukhovaV N Smirnov
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N UekiK Higashino
Jan 1, 1987·Differentiation; Research in Biological Diversity·O Kocher, G Gabbiani
Jan 1, 1987·Differentiation; Research in Biological Diversity·U Hedin, J Thyberg
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M K OwadaS Kakiuchi
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·M GimonaD M Helfman
Sep 15, 1995·The Journal of Biological Chemistry·C H StreuliC J Watson
Jun 1, 1993·Molecular and Cellular Biology·M MuthuchamyD F Wieczorek
May 1, 1995·The Journal of Cell Biology·K S WarrenJ J Lin
Jun 2, 1995·The Journal of Biological Chemistry·J SolwayM S Parmacek
Nov 30, 1993·Biochemical and Biophysical Research Communications·M HarunaK Sobue
Feb 1, 1993·Current Opinion in Cell Biology·F Matsumura, S Yamashiro

❮ Previous
Next ❯

Citations

Jul 26, 2008·World Journal of Urology·Dave ShuklaDarren R Tyson
Aug 5, 2005·Annals of Biomedical Engineering·Gordon M RihaChangyi Chen
Mar 22, 2002·The International Journal of Biochemistry & Cell Biology·Adeeb A GirjesJulie H Campbell
Nov 1, 2002·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·L BianconeF Pallone
Nov 3, 1998·Immunology and Cell Biology·C DufourP Gunning
Aug 17, 2012·American Journal of Physiology. Renal Physiology·Tanchun WangRobert S Moreland
Feb 26, 2014·The Journal of Cell Biology·Tsuyoshi Morita, Ken'ichiro Hayashi
Oct 11, 2001·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·M IijimaK Momose
May 22, 2003·International Journal of Urology : Official Journal of the Japanese Urological Association·Seiji MatsumotoTakashi Kurita
Feb 10, 2006·Tissue & Cell·Laura PolisenoGiuseppe Rainaldi
Apr 8, 2009·International Review of Cell and Molecular Biology·Jim Jung-Ching LinJian-Ping Jin
Dec 14, 2006·Prostaglandins & Other Lipid Mediators·Kristina M FetalveroJohn Hwa
Jul 13, 2007·Molecular Aspects of Medicine·Luis VillacortaJean-Marc Zingg
Oct 8, 2003·The Journal of Biological Chemistry·Qi Quan HuangFrank V Brozovich
Jun 9, 2004·The Journal of Biological Chemistry·Sebastian AlbinssonPer Hellstrand
Jul 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·J M LemireT N Wight
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Qi Jun ZhangMartin Bennett
Sep 9, 2006·The Journal of Biological Chemistry·Stéphanie PasquetNadine Thézé
Oct 23, 1997·The Journal of Biological Chemistry·H ObataK Sobue
Mar 21, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Randolph L CortelingDonald G Welsh
May 9, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Y H ArensK E Kamm
Mar 11, 2000·American Journal of Physiology. Cell Physiology·W P DirksenS A Fisher
May 1, 2012·Journal of Cell Science·Sari TojkanderPekka Lappalainen
Jun 27, 1998·Biochemical and Biophysical Research Communications·K IijimaY Ouchi
Feb 3, 1998·Biochemical and Biophysical Research Communications·T MomiyamaK Sobue
Oct 30, 2003·European Journal of Cell Biology·Marouan AbouhamedGabriele Plenz

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.