Abstract
The purpose of this study was to determine the localization of bone morphogenetic protein-2 (BMP-2), BMP receptors (BMPRs) and Noggin in mouse spinal tissues. The coordinate expression of these positive and negative regulators of BMP signaling may elucidate regulatory mechanisms for bone induction in the spine. Whole spines from 3-week-old mice were used and the spatial expression profiles of BMP-2, BMPR-1a, -1b, -2 and Noggin were examined using in situ hybridization. BMP-2, BMPR-1b and -2 were observed in bone marrow cells in the vertebrae, chondrocytes, hyaline cartilage cells and fibrous cells in the intervertebral discs and neurons of the spinal cord in the entire spine. BMPR-1a was also observed in these cells, but only in the cervical spine. Noggin was expressed in bone marrow cells in the vertebrae, chondrocytes and hyaline cartilage cells and fibrous cells in the intervertebral discs in the entire spine and in neurons in the spinal cord in the cervical and thoracic regions. Noggin was also expressed in the anterior longitudinal, posterior longitudinal and yellow ligaments in the cervical spine, and in the fibrous cells in the anterior longitudinal and yellow ligaments of the lumbar spine.
References
Jan 1, 1993·Growth Factors·K MiyazonoC H Heldin
Nov 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y IshidouT Sakou
Aug 23, 1996·Cell·L B ZimmermanR M Harland
Aug 23, 1996·Cell·S A HolleyE L Ferguson
Jun 17, 1998·Bone·T OnishiT Sakou
Dec 17, 1998·The Journal of Clinical Investigation·E GazzerroE Canalis
Jul 29, 1999·Spine·R TakaeT Sakou
Nov 17, 1999·Molecular Biology of the Cell·M FujiiK Miyazono
Jan 8, 2000·Spine·K NishidaC H Evans
Oct 18, 2000·Bone·H KanekoY Hakeda
Jan 9, 2001·Journal of Neurosurgery·T NakaseH Yoshikawa
Feb 13, 2001·Neuron·D A LimA Alvarez-Buylla
May 9, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y YoshimuraK Takaoka
Jun 12, 2002·The Journal of Cell Biology·Ming ZhaoDi Chen
Jan 25, 2003·The Journal of Craniofacial Surgery·Yasuhito ShimakuraEijyu Uchinuma
Mar 6, 2003·Developmental Biology·William A GomesJohn A Kessler
Apr 8, 2003·Journal of Spinal Disorders & Techniques·Jun TakahashiKunio Takaoka
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yukio NakamuraKunio Takaoka
Citations
Jul 10, 2007·Cell and Tissue Research·Jae-Young KimHan-Sung Jung
Feb 5, 2008·Spine·Vivek Joseph, Yoga Raja Rampersaud
Jan 27, 2016·Arthritis Research & Therapy·Samantha C W ChanBenjamin Gantenbein
Apr 22, 2008·Human Mutation·May TassabehjiRaymond A Clarke
Oct 19, 2007·International Journal of Cancer. Journal International Du Cancer·Christian BuschMatthias Oppitz
May 22, 2016·Seminars in Cell & Developmental Biology·Geoffrey J KerrCheryle A Séguin
Mar 4, 2014·Theriogenology·Elina V GarcíaDora C Miceli
Oct 22, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaodan YuYasuhiko Kawakami