Coordinate induction of two antibiotic genes in tracheal epithelial cells exposed to the inflammatory mediators lipopolysaccharide and tumor necrosis factor alpha.

Infection and Immunity
J P RussellC L Bevins

Abstract

Peptides with potent broad-spectrum antibiotic activity have been identified in many animal species. Recent investigations have demonstrated that epithelial cells are a site of antibiotic peptide expression, suggesting that these peptides contribute to host defense at mucosal surfaces. Expression of tracheal antimicrobial peptide (TAP), a member of the beta-defensin family of peptides, is inducible in cultured tracheal epithelial cells (TEC) upon challenge with bacterial lipopolysaccharide (LPS) (G. Diamond, J.P. Russell, and C.L. Bevins, Proc. Natl. Acad. Sci. USA, in press). In this study, an anchored reverse transcriptase PCR strategy was used to determine if TAP was the sole beta-defensin isoform expressed upon stimulation of the cells with LPS. In addition to TAP, a second class of cDNA clones which encoded lingual antimicrobial peptide (LAP), a beta-defensin peptide recently isolated from a different mucosal site, the bovine tongue, was identified (B.S. Schonwetter, E.D. Stolzenberg, and M. Zasloff, Science 267:1645-1648, 1995). Northern (RNA) blot analysis demonstrated in vivo expression of LAP mRNA in tracheal mucosa. Levels of LAP mRNA were higher in cultured TEC challenged with either LPS or tumor necrosis factor alph...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1992·Infection and Immunity·P B EisenhauerR I Lehrer
Aug 1, 1992·The Journal of Cell Biology·M E SelstedA J Ouellette
Feb 1, 1992·Current Opinion in Immunology·M Zasloff
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jan 1, 1995·Annual Review of Immunology·H G Boman
Aug 1, 1995·Journal of Leukocyte Biology·E MartinR I Lehrer
Mar 17, 1995·Science·B S SchonwetterM A Zasloff
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins

❮ Previous
Next ❯

Citations

Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·E D StolzenbergM Zasloff
Oct 6, 1998·Journal of Dental Research·B Henderson, M Wilson
Aug 3, 2000·The Journal of Biological Chemistry·H P JiaP B McCray
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M G ScottR E Hancock
Jul 11, 2001·Critical Care Medicine·L SteinstraesserS C Wang
Feb 5, 2002·Annual Review of Physiology·Brian C Schutte, Paul B McCray
Nov 8, 2002·Respirology : Official Journal of the Asian Pacific Society of Respirology·Tetsuji TomitaYasuyoshi Ouchi
Feb 12, 2004·Oral Microbiology and Immunology·P PremratanachaiJ M Guthmiller
Sep 13, 2006·Veterinary Pathology·B GruborM R Ackermann
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·P FehlbaumG M Anderson
Jan 25, 2002·Nature·Michael Zasloff
Sep 19, 2002·Periodontology 2000·Beverly A Dale
Jun 15, 1999·Pediatric Research·K M Huttner, C L Bevins
Nov 14, 2001·Infection and Immunity·M E KlutJ C Hogg
Mar 29, 2008·Immunogenetics·Paul CormicanCliona O'Farrelly
Jul 12, 2007·Seminars in Immunopathology·Katherine Radek, Richard Gallo
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·S YarusG Diamond
Jul 28, 2001·European Journal of Gastroenterology & Hepatology·K Fellermann, E F Stange
Sep 1, 2005·Immunogenetics·Katja LuenserArne Ludwig
Jun 13, 2003·Folia Microbiologica·P SímaK Sigler
Dec 10, 2002·Hybridoma and Hybridomics·Shigeharu KurosawaHiroshige Sasahara
Mar 20, 2001·Infection and Immunity·C ZhaoR I Lehrer
Jan 5, 2002·Clinical and Diagnostic Laboratory Immunology·Amanda J Fales-WilliamsMark R Ackermann
Feb 7, 2001·The Journal of Biological Chemistry·K LugardonM H Metz-Boutigue
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·P K SinghP B McCray
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M G ScottM R Gold
Jun 19, 2001·Biological Chemistry·W van 't HofA V Amerongen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.