PMID: 15223811Jun 30, 2004Paper

Coordinate signaling by Src and p38 kinases in the induction of cortical cataracts

Investigative Ophthalmology & Visual Science
Jian Zhou, A Sue Menko

Abstract

The goals of this study were to determine whether MAP kinase signaling pathways play a role in the formation of lens cataracts and to examine the potential signaling relationship between Src and MAP kinases in signaling the induction of lens opacities. Embryonic day (E)10 chick lenses were cultured in Medium 199 containing 10% fetal bovine serum. The activation state of Src kinases and the MAP kinases extracellular signal-regulated protein kinase (ERK), c-jun N-terminal kinase (JNK), and p38 in the lens epithelium was determined over a time course from 10 minutes to 10 days in culture by immunoblot analysis. Src kinase activation was suppressed by exposure to the Src family kinase-specific inhibitor PP1. To examine the role of specific MAP kinases in the development of lens opacities, lenses were grown for 10 days in the presence or absence of inhibitors of ERK (U0126), JNK (SP600125), and p38 (SB203580). Lenses were observed and photographed daily, and the degree of opacification was quantified by using image-analysis software. Within a short time after placing embryonic lenses in culture conditions that induce the formation of cataracts, there occurred a great increase in the activation state of the MAP kinase ERK. Activation...Continue Reading

Citations

Nov 24, 2007·Seminars in Cell & Developmental Biology·P S Zelenka, P Arpitha
Jan 16, 2007·Seminars in Cell & Developmental Biology·Anne E Griep
Jan 31, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Michelle LeonardA Sue Menko
Jan 23, 2019·Metabolic Syndrome and Related Disorders·Şahbender Koç, Sadettin Selçuk Baysal
Jul 4, 2009·[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·Xing-ru ZhangAn-guo Sun
Nov 4, 2004·Experimental Biology and Medicine·Amy C LongJoshua A Bomser
Sep 13, 2013·Development·Catherine ChengXiaohua Gong

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