Coordinated expression of cytoskeleton regulating genes in the accelerated neurite outgrowth of P19 embryonic carcinoma cells

Experimental Cell Research
Yoel Bogoch, Michal Linial

Abstract

The embryonal carcinoma P19 cells provide a model to study neuronal differentiation. Cells that are exposed to retinoic acid become mature neurons within a few days with a pronounced axonal and dendritic polarity. Notably, an accelerated rate of neurite extension characterizes densely but not sparsely plated cells. DNA microarray experiments show maximal differences in gene expression of the dense compared to sparse plated cultures at 18 h after plating. The differentially expressed genes are enriched by functions of cell adhesion and cytoskeletal regulation. Doublecortin, Lis1, Reelin, Map2 and dozens of proteins that regulate cytoskeleton dynamics increase in concordance with a rapid neurite extension. A brief elevation in intracellular cAMP via PKA is sufficient to instigate the phenotype of accelerated neurite extension with no effect on P19 cell fate. Furthermore, we show that the cAMP dependent changes in the expression of cytoskeleton regulators such as doublecortin are restricted to a short time window prior to the establishment of functional neurons. We propose that the wave of gene expression of cytoskeletal regulators that is accompanied by accelerated neurite extension acts in remodeling young developing neurons in ...Continue Reading

References

Aug 1, 1982·The Journal of Cell Biology·E M Jones-VilleneuveV I Kalnins
Nov 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D Parnas, M Linial
Jul 18, 1997·Brain Research. Developmental Brain Research·D Parnas, M Linial
May 20, 1998·Journal of Molecular Neuroscience : MN·D ParnasM Linial
Jul 25, 2000·Brain Research. Brain Research Protocols·Y BlediM Linial
Sep 26, 2000·Human Molecular Genetics·M CaspiO Reiner
May 2, 2001·Human Molecular Genetics·O ShmueliO Reiner
Jul 31, 2002·Differentiation; Research in Biological Diversity·Yi WeiGeoffrey Childs
Oct 9, 2002·Current Opinion in Neurobiology·Annette MarkusWilliam D Snider
Apr 2, 2003·Human Molecular Genetics·Mitsuhiro Kato, William B Dobyns
Oct 9, 2003·The Biochemical Journal·Roberto NitschMariastella Zannini
Oct 28, 2003·Nature Genetics·Amir H AssadiGary D Clark
Nov 5, 2003·Journal of Neurobiology·Leif Dehmelt, Shelley Halpain
Nov 5, 2003·Journal of Neurobiology·Phillip R Gordon-Weeks
Jan 27, 2004·Neuron·Joseph LoTurco
Feb 7, 2004·The EMBO Journal·Amos GdalyahuOrly Reiner
May 1, 2004·Cell Cycle·Orly ReinerTamar Sapir
Nov 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Charles L LimoliJohn R Fike
Dec 21, 2004·Nucleic Acids Research·H ParkinsonA Brazma
Feb 15, 2005·Experimental Cell Research·Yan YangWayne N Frankel
Jun 21, 2005·Nature Cell Biology·Jun LiJohn A Cooper
Mar 16, 2006·Current Opinion in Neurology·Pierre Gressens
Apr 28, 2006·Molecular Biology of the Cell·Chen WangNaihe Jing
May 25, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Kimberly Gerrow, Alaa El-Husseini
Jun 30, 2006·Journal of Neurochemistry·Angel BarcoEric R Kandel
Apr 6, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tara L WalkerPerry F Bartlett

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