Coordinated expression of the vitronectin receptor and the urokinase-type plasminogen activator receptor in metastatic melanoma cells

The Journal of Clinical Investigation
J NipP Brodt

Abstract

Integrin alpha v beta 3 is a marker of progression in malignant melanoma. Previously we reported that human melanoma cells derived from regional lymph node metastases had increased alpha v beta 3-mediated adhesion to lymph node vitronectin. In the present study, the expression and function of alpha v beta 3 were further investigated with emphasis on the functional relationship between alpha v beta 3 and the urokinase-type plasminogen activator system of proteolysis. We found that metastases-derived melanoma MeWo LNI 6I (6I) and MIM/8 LNI cells had a markedly increased expression of alpha v mRNA transcripts relative to the parent lines which was reflected in significantly elevated levels of the alpha v beta 3 heterodimers on the cell surface. These cells also expressed elevated levels of urokinase plasminogen activator receptor (uPAR) mRNA and had higher levels of surface bound urokinase plasminogen activator as detected by immunolabeling. To determine whether the expression of uPAR and alpha v were linked, alpha v synthesis in the metastatic melanoma cells was suppressed using alpha v antisense phosphorothioate oligonucleotides. This resulted in a marked decrease in detectable alpha v mRNA and protein and a corresponding substr...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Jun 1, 1992·The Journal of Clinical Investigation·B Felding-HabermannD A Cheresh
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A D YurochkoS Haskill
Jan 1, 1992·Annals of Plastic Surgery·D S Rigel
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 20, 1989·JAMA : the Journal of the American Medical Association·A G Glass, R N Hoover
Jan 1, 1986·Annual Review of Biochemistry·L A LiottaU M Wewer
May 1, 1988·DNA·P E DanielsonJ G Sutcliffe
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Mar 15, 1994·International Journal of Cancer. Journal International Du Cancer·S MatteiM Herlyn
Apr 22, 1994·Science·P C BrooksD A Cheresh
Oct 1, 1993·European Journal of Immunology·P Sánchez-MateosF Sánchez-Madrid
Feb 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Blasi
Aug 1, 1993·The Journal of Cell Biology·M S PepperJ D Vassalli

❮ Previous
Next ❯

Citations

Mar 15, 1996·International Journal of Cancer. Journal International Du Cancer·J Heino
May 29, 1996·International Journal of Cancer. Journal International Du Cancer·N J TawilP Brodt
Nov 27, 1996·International Journal of Cancer. Journal International Du Cancer·V GouonN Kieffer
Jun 4, 1998·International Journal of Cancer. Journal International Du Cancer·C N RaoD T Woodley
Nov 26, 2002·International Journal of Cancer. Journal International Du Cancer·Immacolata SilvestriMaria Vincenza Carriero
Mar 30, 2004·Biochemical and Biophysical Research Communications·Nafisa DalviSimon A Whawell
Feb 25, 1998·Biochimica Et Biophysica Acta·M KambeM Hayashi
Mar 10, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P A TownsendM A Horton
Oct 28, 1998·Trends in Cell Biology·J C Porter, N Hogg
Oct 19, 1999·Thyroid : Official Journal of the American Thyroid Association·J W SmitB M Goslings
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I VillanovaA Teti
Aug 17, 2000·Biochemical and Biophysical Research Communications·I C KangK H Chung
Jul 1, 1996·Pathology, Research and Practice·T J de VriesD J Ruiter
Apr 2, 2014·Cell Biology International·Marcelina Elżbieta JanikMałgorzata Przybyło
May 29, 2000·Journal of Gastroenterology and Hepatology·T ItohH Itoh
Jan 23, 2003·Microscopy Research and Technique·Anurag SrivastavaJatinder Kaur
Nov 14, 1998·Annals of Medicine·C M FerrierD J Ruiter
Nov 20, 1997·The Journal of Biological Chemistry·K O SimonL T Duong
Mar 28, 1997·International Journal of Cancer. Journal International Du Cancer·A Stahl, B M Mueller
Nov 9, 2005·Molecular Cancer Therapeutics·Laura BelvisiClaudio Pisano
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Guoqiang ZhangAllison A Eddy
Aug 8, 1998·Trends in Cell Biology·P KeelyR Juliano

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