Coordinated expression of tissue-type plasminogen activator and plasminogen activator inhibitor type 1 during corpus luteum formation and luteolysis in the adult pseudopregnant rat

Endocrinology
K LiuG Selstam

Abstract

Proteolytic activity generated by the plasminogen activator (PA) system is associated with many biological processes. Using an adult pseudopregnant rat model, we have studied how two components of the PA system, tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1), are expressed temporally and spatially during different developmental stages of the corpus luteum (CL). Northern blot analysis, in situ hybridization, in situ zymography, and fibrin overlay were used to analyze the expression and distribution of tPA and PAI-1 messenger RNA (mRNA) as well as PA activity in CL of different ages. We demonstrated that during the luteinization period (approximately days 1-2), tPA mRNA was highly and evenly expressed in newly formed CL, whereas PAI-1 mRNA was mainly detected in the central part of the same CL. In accordance with these findings, proteolytic activity generated by tPA was detected in the outer region of newly formed CL by in situ zymography. During the luteotropic period (approximately days 3-10), tPA mRNA expression was very low. PAI-1 mRNA expression was also low, but increased on day 10. As expected, proteolytic activity was very low during this period. During functional luteolysis (da...Continue Reading

Citations

Sep 11, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Heng ZhangCheng Zhu
Sep 5, 2006·Prenatal Diagnosis·Antonio FarinaTakashi Okai
May 1, 1997·Expert Opinion on Investigational Drugs·P Charlton
Apr 30, 1999·Physiological Reviews·J A McCrackenJ C Lamsa
Jan 27, 2020·Molecular and Cellular Endocrinology·Beili ChenPeter C K Leung
Feb 22, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Saba NikanfarRalf Dittrich

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