Coordinated expression of valine catabolic enzymes during adipogenesis: analysis of activity, mRNA, protein levels, and metabolic consequences

Archives of Biochemistry and Biophysics
N Y KedishviliR A Harris

Abstract

3T3-L1 fibroblasts have limited enzymatic capacity to oxidize valine. Enzymes expressed in these cells allow efficient oxidation of only the first carbon of this branched chain amino acid. The pathway is effectively truncated at the level of 3-hydroxyisobutyrate because of very low expression of two enzymes required for the complete pathway, 3-hydroxyisobutyrate dehydrogenase and methylmalonate semialdehyde dehydrogenase. These two enzymes, as well as the branched chain alpha-ketoacid dehydrogenase, are markedly induced upon differentiation of 3T3-L1 fibroblasts into adipocytes. Flux through the first two decarboxylation steps of valine catabolism is increased dramatically after differentiation, particularly through the step catalyzed by methylmalonate semialdehyde dehydrogenase. Activation of the distal portion of the valine catabolic pathway correlates with significant increases in enzyme protein and mRNA levels for 3-hydroxyisobutyrate dehydrogenase and methylmalonate semialdehyde dehydrogenase, and this establishes the pathway in 3T3-L1 adipocytes for utilization of valine carbon for lipogenesis. The induction profiles of 3-hydroxyisobutyrate dehydrogenase and methylmalonate semialdehyde dehydrogenase are very similar, sugg...Continue Reading

Citations

Jul 10, 2008·Expert Opinion on Drug Metabolism & Toxicology·Satori A MarchittiVasilis Vasiliou
Nov 17, 2015·Nature Chemical Biology·Courtney R GreenChristian M Metallo
Jul 20, 2007·The Journal of Investigative Dermatology·Malgorzata RomanowskaJohn Foerster
Dec 23, 2016·Journal of Pharmaceutical Sciences·Julie TrexelKathleen A Stringer
Oct 1, 2007·Proteomics. Clinical Applications·Mandy ClaessensEdwin C M Mariman
Mar 21, 2013·American Journal of Physiology. Endocrinology and Metabolism·Denise E LackeySean H Adams
May 16, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dipsikha BiswasThomas Pulinilkunnil
Oct 17, 2017·Current Opinion in Clinical Nutrition and Metabolic Care·Daniela Salinas-RubioLilia G Noriega
Jun 27, 2019·Journal of Cellular Physiology·Xia YiWeidong Li
Jan 18, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Mona Synnøve BjuneRolf Kristian Berge
Jan 6, 2007·Journal of Proteome Research·Rachel NavetFrancis E Sluse
Jan 27, 2015·Analytical Chemistry·Catherine E MinogueJoshua J Coon
Nov 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Benoît LabrecqueMarie-France Palin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.