Coordinated gene regulation in the initial phase of salt stress adaptation.

The Journal of Biological Chemistry
Elena Vanacloig-PedrosM Proft

Abstract

Stress triggers complex transcriptional responses, which include both gene activation and repression. We used time-resolved reporter assays in living yeast cells to gain insights into the coordination of positive and negative control of gene expression upon salt stress. We found that the repression of "housekeeping" genes coincides with the transient activation of defense genes and that the timing of this expression pattern depends on the severity of the stress. Moreover, we identified mutants that caused an alteration in the kinetics of this transcriptional control. Loss of function of the vacuolar H(+)-ATPase (vma1) or a defect in the biosynthesis of the osmolyte glycerol (gpd1) caused a prolonged repression of housekeeping genes and a delay in gene activation at inducible loci. Both mutants have a defect in the relocation of RNA polymerase II complexes at stress defense genes. Accordingly salt-activated transcription is delayed and less efficient upon partially respiratory growth conditions in which glycerol production is significantly reduced. Furthermore, the loss of Hog1 MAP kinase function aggravates the loss of RNA polymerase II from housekeeping loci, which apparently do not accumulate at inducible genes. Additionally ...Continue Reading

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Citations

Mar 23, 2017·Molecular Microbiology·Sara Manzanares-EstrederMarkus Proft
Jun 24, 2017·The Journal of Cell Biology·Natsuko JinLois S Weisman
Aug 3, 2016·The Biochemical Journal·M Gomar-Alba, M Del Olmo
Apr 5, 2020·Scientific Reports·Philip G HamillJohn E Hallsworth
Jun 21, 2017·Current Genetics·Amparo Pascual-AhuirMarkus Proft
Nov 11, 2020·International Journal of Molecular Sciences·Amparo Pascual-AhuirMarkus Proft

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