Coordinated nuclear import of RNA polymerase III subunits

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Ulrike Hardeland, Ed Hurt

Abstract

Eukaryotic RNA polymerases are multisubunit assemblies, whose enzymatic function in the nucleus is intensively studied. However, little is known about the biogenesis of the three RNA polymerases and coupling to nucleo-cytoplasmic transport. Here, we show that Rpc128, the second largest subunit of RNA polymerase III, was mislocalized to the cytoplasm, when a short sequence in the N-terminal domain was deleted. Importantly, nuclear import of other, but not all, RNA polymerase III subunits was impaired in this RPC128DeltaN mutant. These data suggest that RNA polymerase III subunits are not imported independently into the nucleus but may require preassembly into cytoplasmic subcomplexes for coordinated nuclear uptake. We expect these studies to be a starting point to dissect the complex biogenesis pathway of eukaryotic RNA polymerases.

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Aug 5, 2004·The Journal of Biological Chemistry·Vikram Govind PanseEd Hurt
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Citations

May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Philippe Cloutier, Benoit Coulombe
Oct 2, 2015·Biochimica Et Biophysica Acta·Dipti Vinayak Vernekar, Purnima Bhargava
Mar 1, 2014·Biochimica Et Biophysica Acta·Małgorzata CieślaMagdalena Boguta
Jan 21, 2012·Trends in Biochemical Sciences·Thomas Wild, Patrick Cramer
Jan 22, 2015·Molecular and Cellular Biology·Małgorzata CieślaMagdalena Boguta
Dec 20, 2017·Molecular Systems Biology·Marie-Therese MackmullMartin Beck
Feb 24, 2017·Open Biology·Ewa Leśniewska, Magdalena Boguta
May 25, 2021·Frontiers in Molecular Biosciences·Ana I Garrido-GodinoFrancisco Navarro
Jun 1, 2021·Frontiers in Molecular Biosciences·Tomasz W Turowski, Magdalena Boguta

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