PMID: 9177903Mar 1, 1997Paper

Coordinated regulation of fibronectin fibril assembly and actin stress fiber formation

Cell Adhesion and Communication
J L Sechler, J E Schwarzbauer

Abstract

Assembly of a fibronectin (FN) matrix is a multistep process which influences a number of cellular functions including intracellular cytoskeletal organization and signaling responses. We have previously reported on a recombinant FN (recFN), FN delta III1-7, which differs from native FN in its rate of fibril formation. To determine the intracellular consequences of a delay in assembly, we compared the distribution of cytoskeletal proteins during the formation of native and recFN matrices by immunofluorescence at various time points. CHO alpha 5 cell cytoskeleton was reorganized in response to both native and recFN matrix formation. Assembly of native FN induced a rapid reorganization of actin into stress fibers and colocalization of alpha 5 beta 1 integrin, focal adhesion kinase (FAK), vinculin, and paxillin to regions of cell-matrix contact. alpha 5 beta 1 integrins and FAK are also clustered upon binding of FN delta III1-7 to cells but actin reorganization and focal adhesion formation are delayed and appear to be dependent on the formation of FN delta III1-7 fibrils. These results suggest that the structural framework of the matrix plays an important role in the ability of FN to initiate intracellular responses.

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Citations

Feb 27, 2004·Biochimica Et Biophysica Acta·Jane Sottile
Feb 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Federica CastellettiMarina Noris
May 24, 2007·Tissue Engineering·Marian PereiraPrabhas V Moghe
Dec 17, 1997·Molecular Biology of the Cell·J L SechlerJ E Schwarzbauer
Jan 14, 2004·Molecular Biology of the Cell·Elizabeth E RobinsonSiobhan A Corbett
Apr 24, 2007·Biochemistry. Biokhimii︠a︡·A V DigtyarS E Severin
Jan 26, 1999·Annals of the New York Academy of Sciences·J L SechlerJ E Schwarzbauer
May 13, 2006·Journal of Thrombosis and Haemostasis : JTH·J Cho, D F Mosher
Aug 3, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Yong Mao, Jean E Schwarzbauer
May 1, 1997·Current Biology : CB·J E Schwarzbauer
Jul 9, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A J HayesJ R Ralphs
Apr 1, 2000·The Journal of Biological Chemistry·D C HockingK J Langenbach
Jul 21, 2007·Circulation Research·Annette SchmidtWilhelm Bloch
Jun 18, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Sheila E FrancisRichard O Hynes
Sep 25, 1998·The Journal of Biological Chemistry·J L Sechler, J E Schwarzbauer
Mar 22, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jung ParkViktor Wixler
Jul 13, 2020·Journal of Biomedical Materials Research. Part a·Carly M GarrisonJean E Schwarzbauer
Mar 29, 2007·The Journal of Biological Chemistry·Sylvia PappMichal Opas
Jul 15, 2003·Journal of Cell Science·Iwona Wierzbicka-Patynowski, Jean E Schwarzbauer

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