Coordinated requirements of human topo II and cohesin for metaphase centromere alignment under Mad2-dependent spindle checkpoint surveillance

Molecular Biology of the Cell
Yusuke Toyoda, Mitsuhiro Yanagida

Abstract

Cohesin maintains sister chromatid cohesion until its Rad21/Scc1/Mcd1 is cleaved by separase during anaphase. DNA topoisomerase II (topo II) maintains the proper topology of chromatid DNAs and is essential for chromosome segregation. Here we report direct observations of mitotic progression in individual HeLa cells after functional disruptions of hRad21, NIPBL, a loading factor for hRad21, and topo II alpha,beta by RNAi and a topo II inhibitor, ICRF-193. Mitosis is delayed in a Mad2-dependent manner after disruption of either or both cohesin and topo II. In hRad21 depletion, interphase pericentric architecture becomes aberrant, and anaphase is virtually permanently delayed as preseparated chromosomes are misaligned on the metaphase spindle. Topo II disruption perturbs centromere organization leading to intense Bub1, but no Mad2, on kinetochores and sustains a Mad2-dependent delay in anaphase onset with persisting securin. Thus topo II impinges upon centromere/kinetochore function. Disruption of topo II by RNAi or ICRF-193 overrides the mitotic delay induced by cohesin depletion: sister centromeres are aligned and anaphase spindle movements occur. The ensuing accumulation of catenations in preseparated sister chromatids may over...Continue Reading

References

Aug 1, 1988·Human Genetics·A de CapoaD A Miller
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·J RocaJ C Wang
Oct 11, 1996·Science·Y Li, R Benezra
Jun 12, 1998·The Journal of Cell Biology·J C WatersE D Salmon
Aug 15, 1998·Trends in Cell Biology·C L Rieder, E D Salmon
Nov 15, 2000·The Journal of Cell Biology·I SumaraJ M Peters
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A SkoufiasR L Margolis
Sep 22, 2001·Current Biology : CB·B M Stern, A W Murray
Feb 21, 2002·Molecular Biology of the Cell·Needhi BhallaAndrew W Murray

❮ Previous
Next ❯

Citations

Dec 10, 2009·Chromosoma·Jonathan M G Higgins
Mar 31, 2010·Chromosoma·Keishi Shintomi, Tatsuya Hirano
Jan 19, 2010·Nature Cell Biology·Raquel A OliveiraKim Nasmyth
Apr 21, 2009·Nature Reviews. Cancer·John L Nitiss
Jun 6, 2009·Nature Reviews. Molecular Cell Biology·Mitsuhiro Yanagida
Feb 5, 2010·Molecular Biology of the Cell·Yasuhiro Kurasawa, Li-yuan Yu-Lee
Jun 6, 2009·Nucleic Acids Research·Mark JohnsonChristine J Farr
Sep 4, 2008·Genes & Development·Rodrigo BermejoMarco Foiani
Aug 26, 2009·Molecular and Cellular Biology·Jennifer RhodesA Thomas Look
Nov 6, 2009·Annual Review of Genetics·Kim Nasmyth, Christian H Haering
Nov 6, 2009·Annual Review of Genetics·Marcin R Przewloka, David M Glover
May 22, 2009·Blood·Justin WrayRobert Hromas
Jan 20, 2011·Journal of Cell Science·Asher CastielShai Izraeli
Aug 30, 2008·PLoS Biology·Paula A CoelhoClaudio E Sunkel
Mar 29, 2007·PloS One·Laura A Díaz-MartínezDuncan J Clarke
Jan 16, 2013·Oncogene·M T Hayashi, J Karlseder
Apr 29, 2008·FEBS Letters·Eunhee Choi, Hyunsook Lee
May 14, 2008·Biochimica Et Biophysica Acta·Ana Losada
Nov 19, 2010·American Journal of Medical Genetics. Part a·Kenichiro YamadaNobuaki Wakamatsu
Aug 11, 2015·Developmental Cell·Rutger C C HengeveldMarcel A T M van Vugt
Jun 9, 2012·Biochimica Et Biophysica Acta·Gunjan D MehtaSantanu Kumar Ghosh
Dec 12, 2012·Current Opinion in Cell Biology·Silvia Remeseiro, Ana Losada
May 14, 2010·The Biochemical Journal·Katherine M FeeneyJoanna L Parish
Sep 20, 2008·Developmental Biology·Ana Pimenta-MarquesRui Gonçalo Martinho
Jun 14, 2014·Frontiers in Oncology·Andrew BurgessSamuel Rogers
Jul 28, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Norihiko NakazawaMitsuhiro Yanagida
Mar 2, 2016·Journal of Cell Science·Valentina SirriPascal Roussel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.