Coordinated RNA-Seq and peptidomics identify neuropeptides and G-protein coupled receptors (GPCRs) in the large pine weevil Hylobius abietis, a major forestry pest

Insect Biochemistry and Molecular Biology
Aniruddha A PanditJulian A T Dow

Abstract

Hylobius abietis (Linnaeus), or large pine weevil (Coleoptera, Curculionidae), is a pest of European coniferous forests. In order to gain understanding of the functional physiology of this species, we have assembled a de novo transcriptome of H. abietis, from sequence data obtained by Next Generation Sequencing. In particular, we have identified genes encoding neuropeptides, peptide hormones and their putative G-protein coupled receptors (GPCRs) to gain insights into neuropeptide-modulated processes. The transcriptome was assembled de novo from pooled paired-end, sequence reads obtained from RNA from whole adults, gut and central nervous system tissue samples. Data analysis was performed on the transcripts obtained from the assembly including, annotation, gene ontology and functional assignment as well as transcriptome completeness assessment and KEGG pathway analysis. Pipelines were created using Bioinformatics tools and techniques for prediction and identification of neuropeptides and neuropeptide receptors. Peptidomic analysis was also carried out using a combination of MALDI-TOF as well as Q-Exactive Orbitrap mass spectrometry to confirm the identified neuropeptide. 41 putative neuropeptide families were identified in H. ab...Continue Reading

Citations

Sep 1, 2019·Archives of Insect Biochemistry and Physiology·Gerd GädeHeather G Marco
Aug 5, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Paweł MarciniakGrzegorz Rosiński
Mar 7, 2019·Frontiers in Endocrinology·Melissa FaddaLiliane Schoofs
May 7, 2020·Frontiers in Endocrinology·Marcel Bläser, Reinhard Predel

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