Coordinated upregulation of alpha 1- and beta II-tubulin mRNAs during collateral axonal sprouting of central peptidergic neurons

Journal of Neuroscience Research
C M PadenM M Oblinger

Abstract

An in situ hybridization study was performed to determine the relationship between levels of mRNAs for the axonal growth-associated alpha 1-tubulin and beta II-tubulin isotypes and the process of collateral axonal sprouting by identified central nervous system (CNS) neurons. A unilateral hypothalamic knife-cut was used to hemisect the hypothalamoneurohypophysial tract, which results in a robust collateral sprouting response by the uninjured neurons of the contralateral supraoptic nucleus (SON) (Watt and Paden: Exp Neurol 111:9-24, 1991). At 10 and 30-35 days after the lesion, cryosections of the SON were obtained and hybridized with 35S-labeled cDNA probes specific to alpha 1- and beta II-tubulin mRNAs. Quantitative evaluation of the resulting autoradiographs revealed that alpha 1-tubulin mRNA levels were significantly increased by 10 days in SON neurons that were undergoing collateral sprouting compared to controls and that this increase was sustained at 30-35 days post-lesion. Less marked increases in hybridization intensity of the beta II-tubulin probe were also apparent in sprouting neurons at both 10 and 30-35 days after the lesion, but were statistically significant only at 10 days. The measured increases in intensity of ...Continue Reading

References

Sep 1, 1991·Journal of Neuroscience Research·S A Mikucki, M M Oblinger
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P N Hoffman, D W Cleveland
Sep 1, 1985·The Journal of Cell Biology·S A LewisN J Cowan
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Sep 21, 1984·Science·C W Cotman, M Nieto-Sampedro
Dec 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J T McCabe, R P Bolender
Jan 1, 1993·Journal of Neuroscience Research·P F MoskowitzM M Oblinger
Jan 1, 1993·Brain Research Bulletin·S A Kost, M M Oblinger
Nov 20, 1995·The Journal of Comparative Neurology·C M PadenM M Oblinger

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