Coordinating epidermal growth factor-induced motility promotes efficient wound closure

American Journal of Physiology. Cell Physiology
Richard C KurtenStacie M Jones

Abstract

Wound healing is a response to injury that is initiated to reconstruct damaged tissue. In skin, reepithelialization involves both epithelial cells and fibroblasts and contributes to the reformation of a barrier between the external environment and internal milieu. Growth factors including epidermal growth factor (EGF) play important roles in promoting this process. In the present studies we employed CV-1 fibroblasts in a tissue culture model of reepithelialization to develop strategies for optimizing wound closure stimulated by EGF. We found that EGF enhanced cell motility within 6-8 h of EGF treatment in serum-free medium but wounds failed to close within 24 h. However, if medium on these cultures was exchanged for medium containing serum, cells pretreated with EGF closed new scrape wounds more rapidly than did cells that were not pretreated. These results indicate that serum factors work in concert with EGF to coordinate cell motility for efficient wound closure. Indeed, EGF enhanced the rate of wound closure in the presence of serum, and this effect also persisted for at least 24 h after EGF was removed. This coordination of EGF-induced cell motility was accompanied by an increase in the transient phosphorylation of ERK1 and...Continue Reading

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Citations

Oct 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nir S Gov
Jan 10, 2008·PloS One·Aurore SchneiderChristophe Egles
Oct 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·M PoujadeP Silberzan
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Aug 14, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joseph HardwickeRuth Duncan
May 21, 2010·Physical Review Letters·Thomas E AngeliniDavid A Weitz

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