Coordination chemistry of the heme in cystathionine beta-synthase: formation of iron(II)-isonitrile complexes

Biochemical and Biophysical Research Communications
S Vadon-Le GoffD Mansuy

Abstract

Interaction of rat and human cystathionine-beta-synthase (CBS) with various potential ligands has been studied by visible and EPR spectroscopy in order to explore the coordination chemistry of this atypical hemeprotein. Ferric CBS did not react with any classical hemeprotein ligands, such as various imidazole and pyridine derivatives, N(-)(3) and isonitriles RNC. Ferrous CBS also failed to bind these nitrogenous ligands or nitrosoalkanes. However, it reacts with various isonitriles RNC, leading to complexes characterized by a Soret peak at 433 +/- 2 nm. Binding of isonitriles to ferrous CBS is a relatively slow process; its rate markedly depends on the nature of R. It thus seems that the only exogenous ligands able to bind CBS iron are carbon-centered, very strong heme-Fe(II) ligands such as CNR, CO, and CN(-), presumably after dissociation of the CBS-iron(II)-cysteinate bond. Isonitriles appear as interesting tools for further studies on the topology of CBS active site.

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Citations

Feb 15, 2011·Biochimica Et Biophysica Acta·Sangita Singh, Ruma Banerjee
Sep 8, 2009·Archives of Biochemistry and Biophysics·Laura CelanoBeatriz Alvarez
Jan 26, 2011·Archives of Biochemistry and Biophysics·Tomas MajtanJan P Kraus
Dec 8, 2004·Archives of Biochemistry and Biophysics·Ruma Banerjee, Cheng-Gang Zou
Apr 19, 2005·Journal of Inorganic Biochemistry·Rémy RicouxJean-Pierre Mahy
May 31, 2007·Natural Product Reports·Sangita SinghRuma Banerjee
Feb 13, 2016·The Journal of Biological Chemistry·Sebastián CarballalRuma Banerjee
Nov 8, 2017·Chemical Reviews·Milos R FilipovicRuma Banerjee
Jun 25, 2013·Biochemistry·Sebastián CarballalBeatriz Alvarez
Feb 2, 2021·ACS Omega·Dayana BenchoamBeatriz Alvarez
Jul 2, 2021·Chemical Reviews·Alberto MassarottiGian Cesare Tron

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