Coordination of EZH2 and SOX2 specifies human neural fate decision.

Cell Regeneration
Yuan ZhaoGuangjin Pan

Abstract

Polycomb repressive complexes (PRCs) are essential in mouse gastrulation and specify neural ectoderm in human embryonic stem cells (hESCs), but the underlying molecular basis remains unclear. Here in this study, by employing an array of different approaches, such as gene knock-out, RNA-seq, ChIP-seq, et al., we uncover that EZH2, an important PRC factor, specifies the normal neural fate decision through repressing the competing meso/endoderm program. EZH2-/- hESCs show an aberrant re-activation of meso/endoderm genes during neural induction. At the molecular level, EZH2 represses meso/endoderm genes while SOX2 activates the neural genes to coordinately specify the normal neural fate. Moreover, EZH2 also supports the proliferation of human neural progenitor cells (NPCs) through repressing the aberrant expression of meso/endoderm program during culture. Together, our findings uncover the coordination of epigenetic regulators such as EZH2 and lineage factors like SOX2 in normal neural fate decision.

References

Jun 8, 2001·Molecular and Cellular Biology·D O'CarrollT Jenuwein
Jan 10, 2002·Genesis : the Journal of Genetics and Development·E M Morin-KensickiT Magnuson
Mar 7, 2007·Molecular and Cellular Biology·Diego PasiniKristian Helin
Sep 10, 2009·Nature Reviews. Molecular Cell Biology·Jeffrey A Simon, Robert E Kingston
May 21, 2010·Nature Reviews. Neuroscience·Yusuke Hirabayashi, Yukiko Gotoh
Sep 2, 2010·Cell Stem Cell·Lauren E SurfaceLaurie A Boyer
Jan 21, 2011·Nature·Raphaël Margueron, Danny Reinberg
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Aug 3, 2012·Cold Spring Harbor Perspectives in Biology·Jennifer Nichols, Austin Smith
May 15, 2013·Molecular and Cellular Biology·Biswarup SahaSoumen Paul
Oct 8, 2013·Nature Structural & Molecular Biology·Luciano Di Croce, Kristian Helin
May 7, 2014·Nucleic Acids Research·Fidel RamírezThomas Manke
Jun 24, 2016·Nature Reviews. Neuroscience·Bing YaoHongjun Song
Aug 16, 2016·Nature Neuroscience·Melanie von SchimmelmannAnne Schaefer
Sep 25, 2017·Cell·Bernd SchuettengruberGiacomo Cavalli
Sep 25, 2017·Nature Communications·Yongli ShanGuangjin Pan

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