COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation

Proceedings of the National Academy of Sciences of the United States of America
Na LianTonglin Mao

Abstract

CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), a well-known E3 ubiquitin ligase, functions as a central regulator of plant growth and photomorphogenic development in plants, including hypocotyl elongation. It has been well-established that, in darkness, COP1 targets many photomorphogenesis-promoting factors for ubiquitination and degradation in the nucleus. However, increasing evidence has shown that a proportion of COP1 is also localized outside the nucleus in dark-grown seedlings, but the physiological function of this localization remains largely unclear. In this study, we demonstrate that COP1 directly targets and mediates the degradation of WAVE-DAMPENED 2-LIKE 3 (WDL3) protein, a member of the microtubule-associated protein (MAP) WVD2/WDL family involved in regulating hypocotyl cell elongation of Arabidopsis seedlings. We show that COP1 interacts with WDL3 in vivo in a dark-dependent manner at cortical microtubules. Moreover, our data indicate that COP1 directly ubiquitinates WDL3 in vitro and that WDL3 protein is degraded in WT seedlings but is abundant in the cop1 mutant in the dark. Consistently, introduction of the wdl3 mutation weakened, whereas overexpression of WDL3 enhanced, the short-hypocotyl phenotype of cop1 mutant i...Continue Reading

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Citations

Dec 19, 2019·Frontiers in Cell and Developmental Biology·Liu XiaoxiaRao Guodong
Nov 2, 2020·Journal of Experimental Botany·Andrei SmertenkoSharol Schmidt
Apr 10, 2021·Frontiers in Plant Science·Fredilson Veiga MeloTiago Filipe Lourenço

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Methods Mentioned

BETA
ubiquitination
two-hybrid
pull-down
pull down
coimmunoprecipitation
Confocal microscopy
bimolecular fluorescence complementation
transgenic
Scanning electron microscopy
Assay

Software Mentioned

ImageJ

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