COP9 signalosome complex subunit 5, an IFT20 binding partner, is essential to maintain male germ cell survival and acrosome biogenesis†.

Biology of Reproduction
Qian HuangZ Zhang

Abstract

Intraflagellar transport protein 20 (IFT20) is essential for spermatogenesis in mice. We discovered that COPS5 was a major binding partner of IFT20. COPS5 is the fifth component of the constitutive photomorphogenic-9 signalosome (COP9), which is involved in protein ubiquitination and degradation. COPS5 is highly abundant in mouse testis. Mice deficiency in COPS5 specifically in male germ cells showed dramatically reduced sperm numbers and were infertile. Testis weight was about one third compared to control adult mice, and germ cells underwent significant apoptosis at a premeiotic stage. Testicular poly (ADP-ribose) polymerase-1, a protein that helps cells to maintain viability, was dramatically decreased, and Caspase-3, a critical executioner of apoptosis, was increased in the mutant mice. Expression level of FANK1, a known COPS5 binding partner, and a key germ cell apoptosis regulator was also reduced. An acrosome marker, lectin PNA, was nearly absent in the few surviving spermatids, and expression level of sperm acrosome associated 1, another acrosomal component was significantly reduced. IFT20 expression level was significantly reduced in the Cops5 knockout mice, and it was no longer present in the acrosome, but remained in...Continue Reading

References

Jan 1, 1991·Annual Review of Cell Biology·D Finley, V Chau
May 28, 1998·The Journal of Biological Chemistry·H Y ChenM L Meistrich
Mar 22, 2001·EMBO Reports·D A Chamovitz, D Segal
Jun 23, 2001·Biology of Reproduction·L D RussellG R MacGregor
Sep 25, 2001·Trends in Cell Biology·C Schwechheimer, X W Deng
Mar 1, 2002·Biology of Reproduction·Zhonglin HaoJohn C Herr
Nov 5, 2002·Nature Reviews. Molecular Cell Biology·Joel L Rosenbaum, George B Witman
Nov 26, 2002·EMBO Reports·Vered Maytal-KivityMichael H Glickman
May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jorma ToppariWei Yan
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Ning Wei, Xing Wang Deng
Jan 23, 2004·PLoS Biology·Xavier I AmbroggioRaymond J Deshaies
Apr 15, 2004·Genes & Development·Lynne BemisWayne Zundel
Jun 26, 2004·Human Reproduction·K K NgD J Handelsman
Aug 10, 2004·The Journal of Biological Chemistry·Kiichiro TomodaJun-Ya Kato
Feb 11, 2005·Archives of Histology and Cytology·Abraham L Kierszenbaum, Laura L Tres
Feb 17, 2006·Genes & Development·Timothy C Hallstrom, Joseph R Nevins
Jul 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gil BornsteinAvram Hershko
Oct 2, 2007·Annals of the New York Academy of Sciences·Timothy J DoyleKwan Hee Kim
Nov 17, 2007·Cellular Signalling·Xiangjun LiuFuchu He
Feb 13, 2008·The Journal of Experimental Medicine·Martina PanattoniRuggero Pardi
Jan 17, 2009·Current Topics in Developmental Biology·Wallace F Marshall
Aug 7, 2009·Journal of Cellular and Molecular Medicine·Lukas OrelJohannes A Schmid
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zhibing ZhangJerome F Strauss
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Chandrima ShahaDurga Prasad Mishra
Oct 20, 2010·Cell Division·Terry J Shackleford, Francois X Claret
Oct 28, 2010·Cellular and Molecular Life Sciences : CMLS·Hailong WangLinfang Wang
Aug 31, 2011·Cell Cycle·Mong-Hong LeeSai-Ching J Yeung
Sep 29, 2011·Molecular Biology Reports·Yun-Shu FanWan-Xi Yang

❮ Previous
Next ❯

Citations

May 28, 2020·International Journal of Molecular Sciences·Maria E TevesPaulene Sapao
Dec 10, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ling ZhangZhibing Zhang
Jun 17, 2021·Biology of Reproduction·Wenfeng XiongZhugang Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis