CopH from Cupriavidus metallidurans CH34. A novel periplasmic copper-binding protein

Biochemistry
Véronique SendraJacques Covès

Abstract

The copH gene is one of the 19 open reading frames (ORFs) found in the cop cluster borne by the large plasmid pMol30 in Cupriavidus metallidurans CH34. The entire cluster is involved in detoxification of copper from the cytoplasm as well as from the periplasm. The function of the corresponding protein, CopH, is not yet clear, but it seems to be involved in the late response phase. We have cloned copH and overproduced and purified the corresponding protein. CopH is rather unique as only one paralog can be found in the databases. It is a dimeric protein with a molecular mass of 13 200 Da per subunit and located in the periplasm. The metal binding properties of CopH were examined by using a series of techniques such as UV-visible spectroscopy, circular dichroism (CD), electron paramagnetic resonance (EPR), surface plasmon resonance (SPR), mass spectrometry, and nuclear magnetic resonance (NMR). All together, the corresponding data are consistent with a dimeric protein containing one metal-binding site per subunit. These sites have a high affinity for Cu(II) but can also bind zinc or nickel. CopH does not contain any cysteines or methionines but contains two histidines. EPR and UV-visible features are consistent with the presence o...Continue Reading

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Citations

Oct 1, 2008·Antonie van Leeuwenhoek·Guillaume PompidorJacques Covès
Apr 25, 2013·Environmental Science & Technology·Kris M HartBrian P Kelleher
Oct 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Guillaume PompidorJacques Covès
Jul 14, 2009·PloS One·Aurélien DeniaudEva Pebay-Peyroula
Dec 2, 2009·HFSP Journal·Lucia BanciSimone Ciofi-Baffoni
Jun 28, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pieter MonsieursNatalie Leys
Jul 20, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·David P Giedroc, Alphonse I Arunkumar
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
May 8, 2007·Chemistry : a European Journal·Thomas SchareinaMatthias Beller
Nov 22, 2007·Biochemical and Biophysical Research Communications·Anthony ZoropoguiJacques Covès
Feb 2, 2010·Biochemistry·Isabelle Petit-HaertleinJacques Covès

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