Copia RNA levels are elevated in dunce mutants and modulated by cAMP.

Nucleic Acids Research
Y D Yun, R L Davis

Abstract

Clones carrying sequences expressed at altered abundance levels in dunce mutants were isolated by differentially screening a genomic library with cDNA probes representing the RNA population from dunce+ flies and the RNA population from dunce mutant flies. These mutants have an elevated cAMP content, so some isolates potentially contain cAMP responsive genes. Two classes of clones were isolated. One class contains genes expressed at a higher steady state abundance level in dunce mutants compared to dunce+ flies and the other contains genes expressed at a lower steady state level in the mutants. The recovery of clones from the differential screen demonstrates that in addition to altering normal behavior, fertility, and cAMP metabolism, dunce mutation confers an alteration in the level of expression of certain genes. The class of clones carrying sequences which are overexpressed in the mutants have been characterized. These clones carry a common repetitive sequence which codes for a 5.5 kb poly(A)+ RNA - the RNA species found to be overexpressed in the mutants. Restriction analysis and hybridization experiments show these repetitive sequences to be members of the copia family of transposable elements. Administration of pharmacolog...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M W Young
Apr 28, 1978·Biochemical and Biophysical Research Communications·R L Davis, J A Kiger
Jun 25, 1985·Nucleic Acids Research·D J Strand, J F McDonald
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W RanJ Campisi
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M R MontminyR H Goodman
Nov 25, 1986·Nucleic Acids Research·F PeronnetM Best-Belpomme
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C N ChenR L Davis
Jan 1, 1987·Developmental Biology·A J DiBenedettoM F Wolfner
May 5, 1982·Journal of Molecular Biology·H E SchwartzM W Young
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A J FlavellG M Rubin
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·E O Aceves-PiñaT P Tully
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B L TempelW G Quinn
Oct 14, 1983·Science·T D Sargent, I B Dawid

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