PMID: 11604323Oct 18, 2001Paper

Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes

Current Opinion in Biotechnology
Alexandra Baer, Jürgen Bode

Abstract

Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.

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