Copper and hypoxia modulate transcriptional and mitochondrial functional-biochemical responses in warm acclimated rainbow trout (Oncorhynchus mykiss)

Environmental Pollution
Ravinder SappalCollins Kamunde

Abstract

To survive in changing environments fish utilize a wide range of biological responses that require energy. We examined the effect of warm acclimation on the electron transport system (ETS) enzymes and transcriptional responses to hypoxia and copper (Cu) exposure in fish. Rainbow trout (Oncorhynchus mykiss) were acclimated to cold (11 °C; control) and warm (20 °C) temperatures for 3 weeks followed by exposure to Cu, hypoxia or both for 24 h. Activities of ETS enzyme complexes I-IV (CI-CIV) were measured in liver and gill mitochondria. Analyses of transcripts encoding for proteins involved in mitochondrial respiration (cytochrome c oxidase subunits 4-1 and 2: COX4-1 and COX4-2), metal detoxification/stress response (metallothioneins A and B: MT-A and MT-B) and energy sensing (AMP-activated protein kinase α1: AMPKα1) were done in liver mitochondria, and in whole liver and gill tissues by RT-qPCR. Warm acclimation inhibited activities of ETS enzymes while effects of Cu and hypoxia depended on the enzyme and thermal acclimation status. The genes encoding for COX4-1, COX4-2, MT-A, MT-B and AMPKα1 were strongly and tissue-dependently altered by warm acclimation. While Cu and hypoxia clearly increased MT-A and MT-B transcript levels in...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·R A Capaldi
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Dec 9, 1998·International Journal of Radiation Oncology, Biology, Physics·J A RaleighM R Horsman
May 20, 1999·Annual Review of Pharmacology and Toxicology·C D KlaassenS Choudhuri
Apr 20, 2001·Gene·M HüttemannL I Grossman
Jan 25, 2002·Cell Stress & Chaperones·K A Van Cleef-ToedtJ F Crivello
Apr 12, 2002·Gene·Antoni BarrientosAlexander Tzagoloff
Nov 29, 2002·The Journal of Experimental Biology·Nicolas R BuryChris N Glover
Dec 4, 2003·Mutation Research·Farzana HaqJames Koropatnick
Jun 12, 2004·Biological Reviews of the Cambridge Philosophical Society·Helga Guderley
Mar 18, 2005·The Journal of Biological Chemistry·Louise D McCulloughGabriele V Ronnett
May 17, 2005·Current Medicinal Chemistry·M ValkoM T D Cronin
Aug 23, 2005·Molecular Aspects of Medicine·Janet Y Uriu-Adams, Carl L Keen
Feb 8, 2006·Biochemical and Biophysical Research Communications·Rie UenishiShinji Koizumi
Apr 29, 2006·The Journal of Physiology·D Grahame HardieJohn W Scott
Jun 21, 2006·Biochimica Et Biophysica Acta·Kuppusamy Balamurugan, Walter Schaffner
Nov 9, 2006·Journal of Bioenergetics and Biomembranes·Youfen LiYidong Bai
Mar 3, 2007·Clinical Chemistry·Antoon J M JanssenRichard J T Rodenburg
May 31, 2007·Environmental Science & Technology·Fabien PierronJean-Charles Massabuau
Aug 31, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Fernanda Garcia SampaioFrancisco Tadeu Rantin
Sep 15, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paul M CraigGrant B McClelland
May 7, 2008·IUBMB Life·Darryl Horn, Antoni Barrientos
Aug 16, 2008·Science·Robert J Diaz, Rutger Rosenberg
Sep 23, 2008·The Journal of Experimental Biology·Lindsay A Jibb, Jeffrey G Richards
Oct 10, 2008·Molecular Pharmacology·Wenke FengY James Kang
Oct 17, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kåre-Olav StensløkkenJarle Vaage
Aug 25, 2009·Nutrition Research·Margaret A Broderius, Joseph R Prohaska

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Citations

May 23, 2020·Journal of Fish Biology·Alexander G LittleFrank Seebacher
Jun 9, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Luca PeruzzaChris Hauton
Jun 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Michael O IseiCollins Kamunde

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