Copper-caused oxidative stress triggers the activation of antioxidant enzymes via ZmMPK3 in maize leaves

PloS One
Jianxia LiuRiyu Wen

Abstract

Copper (Cu) is a necessary trace element participated in many physiological processes in plants. But excessive Cu2+ is toxic, which can activate intracellular signals that lead to cellular damage. The mitogen-activated protein kinase (MAPK) cascade is at the center of cell signal transduction and has been reported to be involved in stress-related signaling pathways. ZmMPK3, a kind of MAPKs in maize cells, can be activated by diverse abiotic stresses. In the present study, we investigated the effects of Cu2+ on hydrogen peroxide (H2O2) level, ZmMPK3 activity as well as the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbic acid peroxidase (APX) using maize leaf as an experimental model. The results demonstrated that acute Cu2+ exposure for 24 hours led to rapid increases of H2O2 level and the increase in ZmMPK3 activity as well as the total activities of antioxidant enzymes SOD, CAT and APX. H2O2 scavenger, dimethylthiourea (DMTU), effectively inhibited the Cu2+-increased H2O2 level and the activity of ZmMPK3 as well as the activities of the antioxidant enzymes SOD, CAT and APX. Pre-treatment with the MAPK inhibitor, PD98059, significantly blocked the Cu2+-increased activities of ZmMPK3, CA...Continue Reading

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Citations

May 6, 2019·Environmental Science and Pollution Research International·Muzammal RehmanDingxiang Peng
Dec 28, 2019·International Journal of Molecular Sciences·Muhammad KamranJen-Tsung Chen
Apr 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anju SinghShrikant Kukreti
Feb 26, 2019·Frontiers in Plant Science·Kondeti SubramanyamEls J M Van Damme
Jun 30, 2019·International Journal of Molecular Sciences·Ewa Muszyńska, Mateusz Labudda
Jan 7, 2021·Environmental Science and Pollution Research International·Hamid Reza KavousiMahmoud Ghorbanzadeh Neghab
Feb 9, 2021·Plant Physiology and Biochemistry : PPB·Tauan Rimoldi TavantiAndré Rodrigues Dos Reis
May 8, 2021·Frontiers in Microbiology·Andrea FerrariniEdoardo Puglisi
Jul 7, 2021·Physiologia Plantarum·Ravneet KaurHasthi Ram
Oct 8, 2021·The New Phytologist·Yang XiangAying Zhang

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Methods Mentioned

BETA
immunoprecipitation

Software Mentioned

Image J
SPSS

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