Copper distributed by Atx1 is available to copper amine oxidase 1 in Schizosaccharomyces pombe.

Eukaryotic Cell
Chardeen PeterS Labbé

Abstract

Copper amine oxidases (CAOs) have been proposed to be involved in the metabolism of xenobiotic and biogenic amines. The requirement for copper is absolute for their activity. In the fission yeast Schizosaccharomyces pombe, cao1(+) and cao2(+) genes are predicted to encode members of the CAO family. While both genes are expressed in wild-type cells, we determined that the expression of only cao1(+) but not cao2(+) results in the production of an active enzyme. Site-directed mutagenesis identified three histidine residues within the C-terminal region of Cao1 that are necessary for amine oxidase activity. By use of a cao1(+)-GFP allele that retained wild-type function, Cao1-GFP was localized in the cytosol (GFP is green fluorescent protein). Under copper-limiting conditions, disruption of ctr4(+), ctr5(+), and cuf1(+) produced a defect in amine oxidase activity, indicating that a functionally active Cao1 requires Ctr4/5-mediated copper transport and the transcription factor Cuf1. Likewise, atx1 null cells exhibited substantially decreased levels of amine oxidase activity. In contrast, deletion of ccc2, cox17, and pccs had no significant effect on Cao1 activity. Residual amine oxidase activity in cells lacking atx1(+) can be restor...Continue Reading

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Citations

Aug 11, 2011·The Journal of Biological Chemistry·Jude BeaudoinSimon Labbé
Mar 9, 2010·Annual Review of Biochemistry·Nigel J Robinson, Dennis R Winge
Aug 10, 2010·PloS One·Raphaël IoannoniSimon Labbé
May 5, 2011·World Journal of Biological Chemistry·Simon Labbé
Feb 27, 2014·The Journal of Biological Chemistry·Samuel PlanteSimon Labbé
Nov 22, 2013·Biochemical Society Transactions·Jude BeaudoinSimon Labbé
Sep 10, 2017·Annual Review of Microbiology·Aaron D SmithDennis J Thiele
Apr 4, 2021·Journal of Fungi·Janelle R RobinsonFelicia N Anike

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