Copper iodide staining of protein blots on nitrocellulose membranes.

Analytical Biochemistry
D D Root, E Reisler

Abstract

Copper iodide staining which can detect protein levels as low as 100-150 pg/mm2 on nitrocellulose membranes is described. The staining is quantitative as measured by densitometry. Staining is complete within 5 min and may be removed by washing the membrane for 15 min without loss of immunoreactivity. The stain utilizes a reddish-brown precipitate of copper iodide in highly alkaline conditions. Because of its high sensitivity, convenience, and low cost, this stain may be more practical than amido black or gold- and silver-based stains for most laboratory purposes.

References

Jun 20, 1986·Journal of Chromatography·J F HollandC C Sweeley
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Feb 1, 1983·Analytical Biochemistry·W Lin, H Kasamatsu
May 1, 1983·Analytical Biochemistry·J M Gershoni, G E Palade
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon

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Citations

Jan 1, 1992·Journal of Neuroimmunology·J B VentimigliaD D Bigner
Apr 1, 1990·Analytical Biochemistry·D D Root, E Reisler
Sep 23, 2006·Proteomics·Ingrid MillerElisabetta Gianazza
Apr 18, 2000·Electrophoresis·H Y HongJ K Choi
Nov 13, 2004·Proteomics·Richard Barry, Mikhail Soloviev

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