Aug 25, 2009

Copper oxide nanoparticles induce oxidative stress and cytotoxicity in airway epithelial cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Baher Fahmy, Stephania A Cormier

Abstract

Metal oxide nanoparticles are often used as industrial catalysts and elevated levels of these particles have been clearly demonstrated at sites surrounding factories. To date, limited toxicity data on metal oxide nanoparticles are available. To understand the impact of these airborne pollutants on the respiratory system, airway epithelial (HEp-2) cells were exposed to increasing doses of silicon oxide (SiO(2)), ferric oxide (Fe(2)O(3)) and copper oxide (CuO) nanoparticles, the leading metal oxides found in ambient air surrounding factories. CuO induced the greatest amount of cytotoxicity in a dose-dependent manner; while even high doses (400 microg/cm(2)) of SiO(2) and Fe(2)O(3) were non-toxic to HEp-2 cells. Although all metal oxide nanoparticles were able to generate ROS in HEp-2 cells, CuO was better able to overwhelm antioxidant defenses (e.g. catalase and glutathione reductase). A significant increase in the level of 8-isoprostanes and in the ratio of GSSG to total glutathione in cells exposed to CuO suggested that ROS generated by CuO induced oxidative stress in HEp-2 cells. Co-treatment of cells with CuO and the antioxidant resveratrol increased cell viability suggesting that oxidative stress may be the cause of the cyto...Continue Reading

Mentioned in this Paper

Saponin
Copper dioxide (CuO2)
Penicillamine
Lung
Enzymes, antithrombotic
Glutathione Disulfide
Squamous Transitional Epithelial Cell Count
Protoplasm
Tetrazolium Salts
Alveolar

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