Copper regulates the interactions of antimicrobial piscidin peptides from fish mast cells with formyl peptide receptors and heparin

The Journal of Biological Chemistry
So Young KimMyriam L Cotten

Abstract

Phagocytic cells in fish secrete antimicrobial peptides (AMPs) such as piscidins, glycosaminoglycans such as heparin, and copper ions as first-line immune defenses. Recently, we established that Cu2+ coordination by piscidins 1 (P1) and 3 (P3) enhances their antibacterial activity against membranes and DNA. Interestingly, we noted that physicochemical similarities exist between both piscidins and other AMPs that interact with heparin and induce immune-cell chemotaxis through formyl peptide receptors (FPRs) involved in innate immunity. Thus, we postulated that P1 and P3 interact with heparin and FPRs but that these interactions distinctively depend on Cu2+ Here, we investigate the interactome potentiated by piscidins, heparin, FPR, and Cu2+ Utilizing FPR-transfected cells and neutrophils, we demonstrate that both piscidins exclusively use FPR1 and FPR2 to induce chemotaxis and that Cu2+ reduces their chemotaxis induction. P1 is more effective at activating FPR1 than P3 and other known AMP ligands. Furthermore, the expression of Fpr2 on the surface of neutrophils is down-regulated by both peptides. Copper conjugation of the peptides does not further increase down-regulation, suggesting that the conformational changes induced by t...Continue Reading

References

Jan 1, 1992·Annual Review of Neuroscience·B A EipperR E Mains
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A D Cardin, H J Weintraub
Nov 20, 2001·Nature·U Silphaduang, E J Noga
Oct 9, 2002·Molecular Microbiology·Artur SchmidtchenLars Björck
Oct 29, 2002·Trends in Immunology·Yingying LeJi Ming Wang
Jan 17, 2003·The Journal of Biological Chemistry·Rajaâ El MeskiniBetty A Eipper
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna Di NardoRichard L Gallo
Jun 7, 2003·Drug News & Perspectives·Edward J Noga, Umaporn Silphaduang
Mar 11, 2004·European Journal of Biochemistry·Emma AnderssonArtur Schmidtchen
May 28, 2004·Nature Immunology·Lawrence Steinman
Jul 21, 2004·Accounts of Chemical Research·Robert J Linhardt, Toshihiko Toida
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
May 24, 2005·Nature Immunology·Kim A BrogdenMichael Zasloff
Jan 3, 2006·The Journal of Antimicrobial Chemotherapy·W Barańska-RybakA Schmidtchen
Nov 7, 2006·Cytokine & Growth Factor Reviews·Isabelle MigeotteMarc Parmentier
Dec 28, 2006·Diseases of Aquatic Organisms·U SilphaduangE J Noga
Apr 26, 2007·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Walter P JeskeErwin Coyne
Aug 19, 2007·Nature Reviews. Microbiology·Michael R Yeaman, Nannette Y Yount
Nov 28, 2007·Expert Review of Anti-infective Therapy·John D F Hale, Robert E W Hancock
Nov 28, 2007·TheScientificWorldJournal·Nicole C KaneiderChristian J Wiedermann
Nov 27, 2008·The Journal of Biological Chemistry·Marco Scarselli, Julie G Donaldson
Feb 17, 2009·Nature Reviews. Microbiology·Manuel N MeloMiguel A R B Castanho
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gudmundur BergssonNoel G McElvaney
Nov 17, 2009·Developmental and Comparative Immunology·Jone CorralesEdward J Noga
May 14, 2010·The New England Journal of Medicine·Anton Y Peleg, David C Hooper
Oct 13, 2010·Thrombosis Research·Ragnar FlengsrudOle Rasmus Ødegaard
Dec 28, 2010·Biochimica Et Biophysica Acta·Theoharis C TheoharidesDimitrios Kalogeromitros
Aug 31, 2011·International Review of Cell and Molecular Biology·Gabriella Chieffi BaccariRakesh Kumar Rastogi
Dec 17, 2011·Nature Reviews. Drug Discovery·Christopher D FjellGisbert Schneider
Apr 28, 2012·BMC Veterinary Research·Jareeporn RuangsriViswanath Kiron
Aug 4, 2012·The Journal of Biological Chemistry·Bernd BufeFrank Zufall
Oct 3, 2012·PLoS Pathogens·Richard A Festa, Dennis J Thiele
Feb 14, 2013·The FEBS Journal·Amanda WeyersRobert J Linhardt
Feb 15, 2013·The Journal of Investigative Dermatology·Sebastian RakersRalf Paus

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