Copper-specific transcriptional repression of yeast genes encoding critical components in the copper transport pathway.

The Journal of Biological Chemistry
Simon LabbéDennis J Thiele

Abstract

Copper is an essential micronutrient that is toxic in excess. To maintain an adequate yet non-toxic concentration of copper, cells possess several modes of control. One involves copper uptake mediated by genes encoding proteins that play key roles in high affinity copper transport. These include the FRE1-encoded Cu2+/Fe3+ reductase and the CTR1 and CTR3-encoded membrane-associated copper transport proteins. Each of these genes is transcriptionally regulated as a function of copper availability: repressed when cells are grown in the presence of copper and highly activated during copper starvation. Our data demonstrate that repression of CTR3 transcription is exquisitely copper-sensitive and specific. Although copper represses CTR3 gene expression at picomolar metal concentrations, cadmium and mercury down-regulate CTR3 expression only at concentrations 3 orders magnitude greater. Furthermore, copper-starvation rapidly and potently induces CTR3 gene expression. We demonstrate that the CTR1, CTR3, and FRE1 genes involved in high affinity copper uptake share a common promoter element, TTTGCTC, which is necessary for both copper repression and copper-starvation activation of gene expression. Furthermore, the Mac1p is essential for d...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D J Thiele
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A DancisR D Klausner
Jan 1, 1991·Methods in Enzymology·K Köhrer, H Domdey
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E B GrallaJ S Valentine
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P B Zhou, D J Thiele
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Nov 11, 1990·Nucleic Acids Research·W RychlikR E Rhoads
Jul 1, 1988·Molecular and Cellular Biology·D J Thiele
May 10, 1985·Science·D H HamerJ E Lemontt
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, M Ptashne
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Fogel, J W Welch
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T R ButtS T Crooke
Jan 6, 1995·The Journal of Biological Chemistry·R Hassett, D J Kosman
Nov 28, 1994·FEBS Letters·R D Klausner, A Dancis
Jul 1, 1994·Trends in Genetics : TIG·P C Bull, D W Cox
Jan 1, 1995·Annual Review of Nutrition·C D Vulpe, S Packman
Mar 15, 1996·Science·J Kaplan, T V O'Halloran
Apr 1, 1996·Molecular Microbiology·C C AskwithJ Kaplan

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Aug 23, 2006·Journal of Mammary Gland Biology and Neoplasia·Sarah A Lowndes, Adrian L Harris
Apr 12, 2002·Gene·Heinz D Osiewacz
Dec 22, 1999·Trends in Microbiology·S Labbé, D J Thiele
Aug 30, 2002·The International Journal of Biochemistry & Cell Biology·Corina BorghoutsHeinz D Osiewacz
Jul 17, 1998·Current Opinion in Chemical Biology·D R WingeC Srinivasan
Nov 4, 2008·The Journal of Biological Chemistry·L Kent Wood, Dennis J Thiele
Sep 29, 2012·Genome Biology and Evolution·Andrea Hodgins-DavisJeffrey P Townsend
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·F E Angrave, S V Avery
Feb 12, 2013·Eukaryotic Cell·Jude BeaudoinSimon Labbé
Jul 13, 2006·Eukaryotic Cell·Aubrey K DavisBrian Palenik
Apr 16, 2013·Eukaryotic Cell·Jennifer A SchwartzVincent M Bruno
Nov 10, 2005·Eukaryotic Cell·Greg KellerDennis R Winge
Feb 10, 2006·Eukaryotic Cell·Jude Beaudoin, Simon Labbé
Aug 12, 2000·Journal of Bacteriology·P J Riggle, C A Kumamoto
Aug 21, 2013·Molecular and Cellular Biology·Kangzhen DongJulian C Rutherford
Jul 13, 2000·Molecular and Cellular Biology·Y LiuD Liao
Aug 26, 1998·Annual Review of Nutrition·D J Eide
Oct 5, 2010·Metal-based Drugs·Helen H W Chen, Macus Tien Kuo
Feb 16, 2006·PLoS Computational Biology·Rahul SiddharthanErik van Nimwegen
Nov 15, 2008·PLoS Computational Biology·Anshul KundajeChristina Leslie
May 5, 2012·PloS One·Raphael IoannoniSimon Labbe
Mar 7, 2013·Genetics·Martha S Cyert, Caroline C Philpott

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