Copper stress-induced changes in leaf soluble proteome of Cu-sensitive and tolerant Agrostis capillaris L. populations

Proteomics
Elena HegoMichel Mench

Abstract

Changes in leaf soluble proteome were explored in 3-month-old plants of metallicolous (M) and nonmetallicolous (NM) Agrostis capillaris L. populations exposed to increasing Cu concentrations (1-50 μM) to investigate molecular mechanisms underlying plant responses to Cu excess and tolerance of M plants. Plants were cultivated on perlite (CuSO4 spiked-nutrient solution). Soluble proteins, extracted by the trichloroacetic acid/acetone procedure, were separated with 2-DE (linear 4-7 pH gradient). Analysis of CCB-stained gels (PDQuest) reproducibly detected 214 spots, and 64 proteins differentially expressed were identified using LC-MS/MS. In both populations, Cu excess impacted both light-dependent (OEE, cytochrome b6-f complex, and chlorophyll a-b binding protein), and -independent (RuBisCO) photosynthesis reactions, more intensively in NM leaves (ferredoxin-NADP reductase and metalloprotease FTSH2). In both populations, upregulation of isocitrate dehydrogenase and cysteine/methionine synthases respectively suggested increased isocitrate oxidation and enhanced need for S-containing amino-acids, likely for chelation and detoxification. In NM leaves, an increasing need for energetic compounds was indicated by the stimulation of ATPa...Continue Reading

References

Jun 29, 2004·Proteomics·Sébastien VilainThierry Jouenne
May 20, 2005·Journal of Plant Physiology·Ann CuypersJaco Vangronsveld
Aug 30, 2005·Proteomics·Jean-Marc GionChristophe Plomion
Oct 24, 2007·Nature Methods·Lukas KällMichael J MacCoss
Nov 13, 2009·Nucleic Acids Research·Juan Antonio VizcaínoLennart Martens
Dec 4, 2012·Antioxidants & Redox Signaling·Karl Ravet, Marinus Pilon
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob

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Citations

Feb 26, 2019·The Plant Journal : for Cell and Molecular Biology·Paul FourounjianJoachim Messing
Sep 25, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Swapan Kumar RoySun-Hee Woo
Oct 17, 2020·Cell Biology International·Alla YemetsYaroslav Blume

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