Copresence of prostaglandin EP2 and EP3 receptors on gastric enterochromaffin-like cell carcinoid in African rodents

Gastroenterology
Y Naribayashi-InomotoY Kinoshita

Abstract

Prostaglandins (PGs) have important roles in the regulation of gastric acid secretion. The aim of this study was to examine the possible presence of PG receptors on the gastric enterochromaffin-like (ECL) carcinoid of Mastomys natalensis, which might be a useful model of normal ECL cells. A [3H]PGE2 binding experiment was performed by using the ECL tumor membrane, and intracellular signal transduction was studied in the cells. In addition, Northern blot analysis using EP2 and EP3 receptor complementary DNAs was conducted. [3H]PGE2 specifically bound to the tumor cell membrane, and the binding was displaced by various PGs with a potency order of PGE1 = PGE2 > enprostil > PGF2 alpha. Although PGE1 and PGE2 stimulated 5'-cyclic adenosine monophosphate (cAMP) production, neither PGF2 alpha nor enprostil had any effect. On the other hand, all of PGE1, PGE2, PGF2 alpha, and enprostil attenuated the forskolin-induced cAMP production. Moreover, enprostil inhibited histamine release induced by forskolin. However, on pertussis toxin treatment, PGE2 paradoxically enhanced the forskolin-induced increase of cAMP production. Finally, the presence of EP2 and EP3 receptor messenger RNAs was confirmed by RNA blot analysis. The ECL carcinoid tum...Continue Reading

References

Sep 16, 1992·Biochemical and Biophysical Research Communications·H NakataS Hosoda
Jun 14, 1991·Biochemical and Biophysical Research Communications·T ChibaS Hosoda
May 1, 1974·European Journal of Pharmacology·I H Main, B J Whittle
Sep 1, 1982·Proceedings of the Society for Experimental Biology and Medicine·K TakeuchiW Silen
Aug 1, 1993·Gastroenterology·C PrinzG Sachs

❮ Previous
Next ❯

Citations

Aug 28, 1998·British Journal of Pharmacology·E Lindström, R Håkanson
Apr 12, 2000·Journal of Gastroenterology and Hepatology·K TakeuchiK Tashima
Jul 29, 2006·Journal of Gastroenterology and Hepatology·Shigeaki MizunoNorimichi Nemoto
May 8, 2001·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·K TakeuchiA Tanaka
Jul 6, 2000·The Journal of Laboratory and Clinical Medicine·H SuetsuguY Kinoshita
Jun 18, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shinichi KatoKoji Takeuchi
Jan 31, 2003·The Journal of Biological Chemistry·Tatsuya HoshinoTohru Mizushima
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·N EnomotoR G Thurman
Mar 19, 2020·International Journal of Molecular Medicine·Lin-Lin CaiHai-Long Fu
Oct 20, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Youichiro HattoriMasataka Majima
Nov 1, 1998·Journal of Gastroenterology and Hepatology·Koji TakeuchiKim-Ihito Tashima

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.