PMID: 8600455Mar 1, 1996Paper

Copurification of casein kinase II with transcription factor ATF/E4TF3

Nucleic Acids Research
T WadaH Handa

Abstract

We have developed a simple method to purify sequence-specific DNA-binding proteins directly from crude cell extracts by using DNA affinity latex beads. The method enabled us to purify not only DNA-binding proteins, but also their associated proteins. Using beads bearing the ATF/E4TF3 site from the adenovirus E4 gene promoter, a protein kinase activity was copurified with the ATF/E4TF3 family. We found that the kinase interacted with ATF1 in vitro efficiently. The kinase did not bind directly to DNA. The kinase mainly phosphorylated ATF1 on serine 36, which was one of target amino acids for casein kinase (CK) II. Biological features of the kinase were the same as those of CKII and an anti-CKII serum reacted with the kinase, indicating that the kinase was CKII. Moreover, it was clearly shown that one of CKII subunits, the CKII alpha protein bound to glutathione-S-transferase (GST) fusion ATF1 but not GST in vitro. It has been reported that a specific CKII inhibitor, 5,6-dichloro-1-beta-D-ribo-furanosylbenzimidazole (DRB) inhibits transcription by RNA polymerase II [Zandomeni et al., (1986) J. Biol. Chem. 261, 3414-3419]. Taken together, these results suggest that ATF/E4TF3 may recruit the CKII activity to a transcription initiati...Continue Reading

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Citations

Aug 1, 1998·Nucleic Acids Research·Y YamaguchiH Handa
Dec 8, 2006·Journal of Virology·Noriaki YamamotoHiroshi Handa
Mar 24, 2007·Archives of Biochemistry and Biophysics·José R BardalesJ Antonio Villamarín
Feb 27, 2009·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Satoshi SakamotoHiroshi Handa
Jan 24, 2012·Bioorganic & Medicinal Chemistry·Satoshi SakamotoHiroshi Handa
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Feb 7, 1997·The Journal of Biological Chemistry·D LiE G Krebs
Oct 6, 1997·The Journal of Biological Chemistry·C MitchellB Roizman
Jun 19, 2008·Molecular and Cellular Biochemistry·Birgitte B Olsen, Barbara Guerra
Jul 21, 1998·Annals of the New York Academy of Sciences·J J BiekerX Chen
Jan 20, 2005·The Biochemical Journal·Makoto HaseJunichi Sadoshima

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