CopY-like copper inducible repressors are putative 'winged helix' proteins

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Reto PortmannM Solioz

Abstract

CopY of Enterococcus hirae is a well characterized copper-responsive repressor involved in copper homeostasis. In the absence of copper, it binds to the promoter. In high copper, the CopZ copper chaperone donates copper to CopY, thereby releasing it from the promoter and allowing transcription of the downstream copper homeostatic genes of the cop operon. We here show that the CopY-like repressors from E. hirae, Lactococcus lactis, and Streptococcus mutans have similar affinities not only for their native promoters, but also for heterologous cop promoters. CopZ of L. lactis accelerated the release of CopY from the promoter, suggesting that CopZ of L. lactis acts as copper chaperone, similar to CopZ in E. hirae. The consensus binding motif of the CopY-like repressors was shown to be TACAxxTGTA. The same binding motif is present in promoters controlled by BlaI of Bacillus licheniformis, MecI of Staphylococcus aureus and related repressors. BlaI and MecI have known structures and belong to the family of 'winged helix' proteins. In the N- terminal domain, they share significant sequence similarity with CopY of E. hirae. Moreover, they bind to the same TACAxxTGTA motif. NMR analysis of the N-terminal DNA binding domain of CopY of L. ...Continue Reading

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Citations

Oct 1, 2009·Chemical Reviews·Zhen MaDavid P Giedroc
May 12, 2009·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Miriam Moscoso, Ernesto García
Jun 15, 2007·Journal of Bacteriology·Olivier BarréMarc Solioz
Nov 13, 2007·Journal of Bacteriology·David MagnaniMarc Solioz
Jun 26, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Angélica Reyes-JaraMauricio González
Dec 5, 2006·Nature Chemical Biology·Tong LiuDavid P Giedroc
Jan 29, 2013·Nature Chemical Biology·Yue FuDavid P Giedroc
Jul 20, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·David P Giedroc, Alphonse I Arunkumar
Sep 24, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Marc SoliozStefano Mancini
Dec 14, 2011·Current Opinion in Microbiology·Catherine A Wakeman, Eric P Skaar
Jan 8, 2008·Journal of Inorganic Biochemistry·Kristina O PazehoskiCharles T Dameron
Jan 23, 2015·Metallomics : Integrated Biometal Science·Zhong GuoXuesong Sun
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Aug 25, 2012·Microbiology·Corinna Rademacher, Bernd Masepohl
Feb 11, 2015·Infection and Immunity·Michael D L JohnsonJason W Rosch
Oct 8, 2008·The Biochemical Journal·Francesca CantiniMarc Solioz
Dec 21, 2019·The Journal of Biological Chemistry·Karina A Baksh, Deborah B Zamble
Sep 24, 2020·Membranes·Andreea AndreiHans-Georg Koch
May 11, 2017·Essays in Biochemistry·Daiana A CapdevilaDavid P Giedroc
Jan 25, 2021·The Journal of Biological Chemistry·Karina A Baksh, Deborah B Zamble

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