Copy number alteration and uniparental disomy analysis categorizes Japanese papillary thyroid carcinomas into distinct groups.

PloS One
Michiko MatsuseShunichi Yamashita

Abstract

The aim of the present study was to investigate chromosomal aberrations in sporadic Japanese papillary thyroid carcinomas (PTCs), concomitant with the analysis of oncogene mutational status. Twenty-five PTCs (11 with BRAF(V600E), 4 with RET/PTC1, and 10 without mutation in HRAS, KRAS, NRAS, BRAF, RET/PTC1, or RET/PTC3) were analyzed using Genome-Wide Human SNP Array 6.0 which allows us to detect copy number alteration (CNA) and uniparental disomy (UPD), also referred to as copy neutral loss of heterozygosity, in a single experiment. The Japanese PTCs showed relatively stable karyotypes. Seven cases (28%) showed CNA(s), and 6 (24%) showed UPD(s). Interestingly, CNA and UPD were rarely overlapped in the same tumor; the only one advanced case showed both CNA and UPD with a highly complex karyotype. Thirteen (52%) showed neither CNA nor UPD. Regarding CNA, deletions tended to be more frequent than amplifications. The most frequent and recurrent region was the deletion in chromosome 22; however, it was found in only 4 cases (16%). The degree of genomic instability did not depend on the oncogene status. However, in oncogene-positive cases (BRAF(V600E) and RET/PTC1), tumors with CNA/UPD were less frequent (5/15, 33%), whereas tumors w...Continue Reading

References

May 18, 1999·The American Journal of Pathology·S HemmerH Joensuu
Jun 9, 2001·Thyroid : Official Journal of the American Thyroid Association·U Feldt-Rasmussen
Nov 5, 2002·The American Journal of Pathology·Volkert B WreesmannBhuvanesh Singh
Aug 14, 2003·The Journal of Clinical Endocrinology and Metabolism·Chung J HungQuan-Yang Duh
Mar 24, 2004·Cell·Paul T C WanUNKNOWN Cancer Genome Project
Jun 10, 2004·Genes, Chromosomes & Cancer·Volkert B WreesmannBhuvanesh Singh
Jan 15, 2005·Thyroid : Official Journal of the American Thyroid Association·Hedwig RichterHorst Zitzelsberger
Mar 25, 2006·Nature Reviews. Cancer·Tetsuo KondoSylvia L Asa
May 27, 2006·Endocrine-related Cancer·Päivi SiironenCaj Haglund
Nov 8, 2006·Nucleic Acids Research·Maureen E HigginsAlex E Lash
Apr 13, 2007·Endocrine Journal·Atsushi KumagaiShunichi Yamashita
Jul 25, 2008·Thyroid : Official Journal of the American Thyroid Association·E Dillwyn WilliamsGeraldine A Thomas
Dec 11, 2008·The Journal of Clinical Endocrinology and Metabolism·Anoma NelloreMarcia S Brose
Feb 5, 2009·The Journal of Clinical Endocrinology and Metabolism·Haixia GuanMingzhao Xing
Feb 6, 2009·Endocrine Journal·Reiko WatanabeYoshiharu Murata
Feb 28, 2009·Trends in Molecular Medicine·Musaffe TunaGordon B Mills
Sep 4, 2009·Thyroid : Official Journal of the American Thyroid Association·Leighton SteinLesleyann Hawthorn
Sep 23, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ramon V TiuJaroslaw P Maciejewski
Jan 29, 2010·Blood·Christine O'KeefeJaroslaw P Maciejewski
Jun 16, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Annalisa AstolfiGuido Biasco
Jun 29, 2010·Thyroid : Official Journal of the American Thyroid Association·Mingzhao Xing
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Giuseppe VigliettoCarmela De Marco
Apr 27, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hideki Makishima, Jaroslaw P Maciejewski

❮ Previous
Next ❯

Citations

May 22, 2013·Clinical & Developmental Immunology·J MorscioT Tousseyn
Apr 29, 2015·Clinical Endocrinology·Rui JiangWei Song
Sep 9, 2019·Biological Trace Element Research·Yunfeng GuanHongmei Shen
Mar 31, 2021·Histopathology·Tyler JanovitzJustine A Barletta

❮ Previous
Next ❯

Methods Mentioned

BETA
chromosomal aberrations
PCR
Genotyping
chromosomal aberration

Software Mentioned

GTC
Partek Genomics Suite
Birdseed Genotyping Console ( GTC )
CNAG
Affymetrix GeneChip Command Console
Affymetrix
AsCNAR

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.