PMID: 9529090Apr 7, 1998Paper

Copy number of pilus gene clusters in Haemophilus influenzae and variation in the hifE pilin gene

Infection and Immunity
T D ReadM M Farley

Abstract

Brazilian purpuric fever (BPF)-associated Haemophilus influenzae biogroup aegyptius strain F3031 contains two identical copies of a five gene cluster (hifA to hifE) encoding pili similar to well-characterized Hif fimbriae of H. influenzae type b. HifE, the putative pilus tip adhesin of F3031, shares only 40% amino acid sequence similarity with the same molecule from type b strains, whereas the other four proteins have 75 to 95% identity. To determine whether pilus cluster duplication and the hifE(F3031) allele were special features of BPF-associated bacteria, we analyzed a collection of H. influenzae strains by PCR with hifA- and hifE-specific oligonucleotides, by Southern hybridization with a hifC gene probe, and by nucleotide sequencing. The presence of two pilus clusters was limited to some H. influenzae biogroup aegyptius strains. The hifE(F3031) allele was limited to H. influenzae biogroup aegyptius. Two strains contained one copy of hifE(F3031) and one copy of a variant hifE allele. We determined the nucleotide sequences of four hifE genes from H. influenzae biogroup aegyptius and H. influenzae capsule serotypes a and c. The predicted proteins produced by these genes demonstrated only 35 to 70% identity to the three publi...Continue Reading

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Citations

Sep 14, 2002·Infection and Immunity·Guillaume BruantAgnes Rosenau
Mar 16, 2016·PloS One·Jennifer M Kress-BennettBenjamin A Janto
Nov 26, 2003·Infection and Immunity·Sarah W SatolaMonica M Farley
Oct 13, 2001·Infection and Immunity·D L ClemansJ R Gilsdorf
Apr 23, 2004·Infection and Immunity·Janet R GilsdorfBetsy Foxman
Jul 10, 2004·Journal of Clinical Microbiology·I Zafer EcevitJanet R Gilsdorf

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