Copy-out-Paste-in Transposition of IS911: A Major Transposition Pathway

Microbiology Spectrum
M ChandlerGuy Duval-Valentin

Abstract

IS911 has provided a powerful model for studying the transposition of members of a large class of transposable element: the IS3 family of bacterial Insertion Sequences (IS). These transpose by a Copy-out-Paste-in mechanism in which a double-strand IS circle transposition intermediate is generated from the donor site by replication and proceeds to integrate into a suitable double strand DNA target. This is perhaps one of the most common transposition mechanisms known to date. Copy-out-Paste-in transposition has been adopted by members of at least eight large IS families. This chapter details the different steps of the Copy-out-Paste-in mechanism involved in IS911 transposition. At a more biological level it also describes various aspects of regulation of the transposition process. These include transposase production by programmed translational frameshifting, transposase expression from the circular intermediate using a specialized promoter assembled at the circle junction and binding of the nascent transposase while it remains attached to the ribosome during translation (co-translational binding). This co-translational binding of the transposase to neighboring IS ends provides an explanation for the longstanding observation tha...Continue Reading

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Citations

May 18, 2016·Chemical Reviews·Alison B Hickman, Fred Dyda
Jul 29, 2016·Nucleic Acids Research·Laure LavatineBao Ton-Hoang
Jan 20, 2017·Microbiology·Zhongge ZhangMilton H Saier
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Aug 3, 2018·Clinical Microbiology Reviews·Sally R PartridgeSlade O Jensen
Jul 15, 2020·Antimicrobial Agents and Chemotherapy·Yu-Zhang HeJian Sun

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