Cord Blood Cells Responses to IL2, IL7 and IL15 Cytokines for mTOR Expression

Advanced Pharmaceutical Bulletin
Anahita MohammadianHojjatollah Nozad Charoudeh

Abstract

Purpose: Mammalian target of rapamycin (mTOR)is important in hematopoiesis and affect cell growth,differentiation and survival. Although previous studies were identified the effect of cytokines on the mononuclear cells development however the cytokines effect on mTOR in cord blood mononuclear cells was unclear. The aim of this study was to evaluate mTOR expression in cord blood mononuclear and cord blood stem cells (CD34(+) cells) in culture conditions for lymphoid cell development. Methods: Isolation of The mononuclear cells (MNCs) from umbilical cord blood were done with use of Ficollpaque density gradient. We evaluated cultured cord blood mononuclear and CD34(+) cells in presece of IL2, IL7 and IL15 at distinct time points during 21 days by using flow cytometry. In this study, we presented the role of IL2, IL7 and IL15 on the expression of mTOR in cord blood cells. Results: mTOR expression were increased in peresence of IL2, IL7 and IL15 in day 14 and afterword reduced. However in persence of IL2 and IL15 expression of mTOR significantly reduced. mTOR expression in CD34(+) cells decreased significantly from day7 to day 21 in culture. Conclusion: cytokines play important role in mTOR expression during hematopoiesis and develo...Continue Reading

References

Oct 11, 1990·European Journal of Immunology·L S WickerN H Sigal
Aug 18, 2004·Genes & Development·Nissim Hay, Nahum Sonenberg
Oct 6, 2005·Journal of Leukocyte Biology·Rebecca CruzKay K Lee-Fruman
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jonathan M ShillingfordThomas Weimbs
Aug 24, 2007·American Journal of Physiology. Renal Physiology·Thomas Weimbs
Aug 29, 2007·European Journal of Immunology·Amber C Donahue, David A Fruman
Apr 11, 2009·Transplantation Proceedings·A G ContrerasD M Briscoe
Apr 14, 2009·Trends in Immunology·Thomas Weichhart, Marcus D Säemann
Apr 25, 2009·Nature Reviews. Immunology·Angus W ThomsonGiorgio Raimondi
Jun 23, 2009·Nature Reviews. Immunology·Yrina RochmanWarren J Leonard
Oct 1, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M D SäemannT Weichhart
Mar 18, 2011·Blood Cells, Molecules & Diseases·Sara L RohrabaughHal E Broxmeyer
Dec 6, 2011·Annual Review of Immunology·Jonathan D PowellMaureen R Horton
Jun 19, 2012·Cell Stem Cell·Michael R CopleyConnie J Eaves
Jan 29, 2013·Seminars in Immunology·Xiaojin XuRafi Ahmed
Jun 28, 2013·Journal of Cellular Physiology·Hui YangGuangwei Liu
Jan 17, 2015·Trends in Immunology·Diana Saleiro, Leonidas C Platanias
Aug 2, 2016·Advanced Pharmaceutical Bulletin·Balal BrazvanHojjatollah Nozad Charoudeh

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Methods Mentioned

BETA
flow cytometry
FACS

Software Mentioned

Flow

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