Cord blood mesenchymal stem cells propel human dendritic cells to an intermediate maturation state and boost interleukin-12 production by mature dendritic cells.

Immunology
Lieke C J van den BerkRuurd Torensma

Abstract

Pathogen-derived entities force the tissue-resident dendritic cells (DCs) towards a mature state, followed by migration to the draining lymph node to present antigens to T cells. Bone marrow mesenchymal stem cells (MSCs) modulate the differentiation, maturation and function of DCs. In umbilical cord blood an immature MSC population was identified. Remarkably, these immature stem cells modulated DCs in a different way. Marker expression was unchanged during the differentiation of monocytes towards immature DCs (iDCs) when cocultured with cord blood MSC [unrestricted somatic stem cells (USSCs)]. The maturation to mature DCs (mDCs) was enhanced when DCs were co-cultured with USSC, as evidenced by the up-regulation of costimulatory molecules. Endocytosis of dextran by iDCs was hampered in the presence of USSCs, which is indicative for the maturation of iDCs. Despite this maturation, the migration of iDCs cocultured with USSCs appeared to be identical to iDCs cultured alone. However, USSCs increased the migration of mDCs towards CCL21 and boosted interleukin-12 production. So, USSCs mature iDCs, thereby redirecting the antigen-uptake phenotype towards a mature phenotype. Furthermore, DC maturation by lipopolysaccharide (LPS) or USSC...Continue Reading

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Citations

Aug 20, 2010·Human Gene Therapy·Matthew D GriffinBernard P Mahon
Jul 19, 2011·Journal of Biomedical Science·Pei-Min ChenB-Linju Yen
May 25, 2011·World Journal of Stem Cells·Francesco BifariMauro Krampera
Mar 22, 2014·Stem Cells and Development·Lieke C J van den BerkRuurd Torensma
Oct 1, 2009·Stem Cells and Development·Bastiaan J H JansenGosse J Adema
Dec 22, 2011·Current Opinion in Organ Transplantation·Marieke Roemeling-van RhijnMartin J Hoogduijn

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