Cord blood screening for alpha-thalassemia and hemoglobin variants by isoelectric focusing in northern Thai neonates: correlation with genotypes and hematologic parameters

Blood Cells, Molecules & Diseases
Pimlak CharoenkwanTorpong Sanguansermsri

Abstract

We describe the screening of newborns for thalassemia and Hb variants by using isoelectric focusing (IEF) in a population from northern Thailand where hemoglobinopathies are highly prevalent. The report focuses on findings of alpha-thalassemia, Hb E, and other hemoglobin variants, and their correlation with genotypes and hematologic parameters. Two-hundred and seven out of 566 newborns (36.6%) had thalassemia genes or Hb variants. Seventeen different genotypes were found. Nine cases (1.6%) of Hb H disease (five deletional Hb H diseases, two Hb H/Constant Spring diseases, one deletional Hb H disease/Hb E, carrier and one Hb H/Constant Spring disease/Hb E carrier) and one Hb E-beta-thalassemia were identified. IEF could clearly distinguish Hb H diseases and carriers of two alpha-globin gene defects from normal individuals according to the presence of Hb Bart's and its percentage. For carriers of a single alpha-globin gene defect, Hb Bart's was either absent or present in a small amount and was therefore not reliable for screening. The presence of an additional band at the Hb A(2) position in the newborns signified an Hb E carrier. One case of an absent Hb A and a presence of Hb E was identified as Hb E-beta-thalassemia. Two Hb Q-...Continue Reading

Citations

Sep 15, 2010·International Journal of Hematology·Vannarat SaechanSuthat Fucharoen
Dec 18, 2013·Indian Journal of Human Genetics·Viroj Wiwanitkit
Nov 19, 2010·Pediatric Hematology and Oncology·Dong-Zhi Li
May 23, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Sakorn PornprasertKanokwan Jaiping
May 9, 2015·International Journal of Laboratory Hematology·M-Y WuD-Z Li
Mar 1, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Aziz AchouriFabrice Lécuru
Apr 1, 2015·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Xing-Mei XieDong-Zhi Li
Apr 1, 2015·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Sakorn Pornprasert, Manoo Punyamung
Oct 8, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Noppacharn UaprasertPranee Sutcharitchan
Aug 22, 2019·Expert Review of Hematology·Kanjaksha GhoshAnita Nadkarni
Jun 4, 2020·British Journal of Haematology·Suchaya LuewanTheera Tongsong
Mar 26, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Vip ViprakasitVoravarn S Tanphaichitr
Mar 5, 2019·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Dipti UpadhyeRoshan Colah
Aug 10, 2020·Journal of Pediatric Hematology/oncology·Natthawadee NantaPimlak Charoenkwan
Oct 15, 2020·International Journal of Environmental Research and Public Health·Lucky Poh Wah GohPing-Chin Lee

Related Concepts

Umbilical Cord Blood
Hemoglobin E
Hemoglobin H
Abnormal Hemoglobins
Isoelectric Focusing
Alpha-Thalassemia
A gamma-Globin
Congenital Abnormality
Beta-Globins
Cell Differentiation Process

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