PMID: 20126923Feb 4, 2010Paper

Cord factor detection and macroscopic evaluation of mycobacterial colonies: an efficient combined screening test for the presumptive identification of Mycobacterium tuberculosis complex on solid media

Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia
Fernanda Cristina dos Santos SimeãoMaria Alice da Silva Telles

Abstract

The rapid differentiation between Mycobacterium tuberculosis and nontuberculous mycobacteria is fundamental for patients co-infected with tuberculosis and HIV. To that end, we use two methods in our laboratory: detection of cord factor and PCR-restriction enzyme analysis (PRA). The objective of this study was to evaluate the accuracy of a screening test on solid medium as a rapid method for the presumptive identification of M. tuberculosis complex, considering costs and turnover time. A total of 152 strains were submitted to a combined screening test, consisting of the detection of cord factor under microscopy (Ziehl-Neelsen staining) and evaluation of the macroscopic aspect of colonies, as well as to PRA, which was used as the gold standard. Costs were estimated by calculating the price of all of the materials needed for each test. The overall accuracy of cord factor detection alone was 95.4% (95% CI: 90.7-98.1%), and that of the combined screening test was 99.3% (95% CI: 96.4-100%). Cord factor detection costs US$ 0.25, whereas the PRA costs US$ 7.00. Results from cord factor detection are ready in 2 days, whereas PRA requires 4 days to yield results. The presumptive identification of M. tuberculosis using the macroscopic eva...Continue Reading

References

Apr 1, 1966·Journal of Bacteriology·S W GilkersonF Cuthrell
Sep 1, 1993·Journal of Clinical Microbiology·A J Morris, L B Reller

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Citations

Apr 15, 2011·Journal of Clinical Microbiology·Alejandro Sánchez-ChardiMarina Luquin
Jun 5, 2012·Progress in Lipid Research·Jan A VerschoorJohan Grooten
Mar 9, 2010·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Viroj Wiwanitkit
Mar 24, 2018·Journal of Medical Microbiology·Juliana Maira Watanabe PinhataLucilaine Ferrazoli
Apr 25, 2018·Journal of Medical Microbiology·Juliana Maira Watanabe PinhataRosangela Siqueira de Oliveira

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