Cordyceps militaris polysaccharides exerted protective effects on diabetic nephropathy in mice via regulation of autophagy

Food & Function
Dan-Dan ChenHua Lin

Abstract

The present study was designed to investigate the protective effects of Cordyceps militaris polysaccharides (CMP) on STZ-treated DN mice. CMP were identified by FT-IR and HPLC. Diabetic nephropathy (DN) was induced in male C57BL/6 mice by the injection of streptozotocin (STZ, 50 mg kg-1) in citrate buffer on 5 consecutive days. Administration of CMP at 200 and 400 mg kg-1 or irbesartan at 60 mg kg-1 in the STZ-treated mice could prevent the damage caused by STZ. CMP significantly reduced the STZ-induced higher expression of the kidney index, TC, TG, MDA, urinary protein, Scr, and BUN, while it markedly increased the STZ-induced decrease in GSH levels compared with the DN group. Histopathology analysis of the kidney by PAS, Masson, and HE staining confirmed the renal injury induced by STZ and the protective effects of CMP. Transmission electron microscopy (TEM) results confirmed the severe foot process effacement induced by STZ, but CMP treatment inhibited the podocytes' structure defects and ameliorated the function of podocytes. Desmin was measured by immunofluorescence and was related to podocyte injury. The results showed that CMP lessened the expression of desmin induced by STZ. CD68 expression was measured by immunohistoch...Continue Reading

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Citations

Apr 27, 2021·European Journal of Pharmacology·Dandan ChenJunbiao Wu
Aug 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie SunYan Yang
Aug 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Baris AfsarMehmet Kanbay
Oct 30, 2021·Critical Reviews in Food Science and Nutrition·Bing ZhengQiang Yu

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Methods Mentioned

BETA
Protein Assay
infrared spectrometry
light microscopy
PCR
MDA

Software Mentioned

ImageJ

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