Cordyceps sinensis mycelium activates PKA and PKC signal pathways to stimulate steroidogenesis in MA-10 mouse Leydig tumor cells

The International Journal of Biochemistry & Cell Biology
Yung-Chia ChenBu Miin Huang

Abstract

Cordyceps sinensis (CS) mycelium stimulates steroidogenesis in MA-10 mouse Leydig tumor cells, but the mechanisms remain unclear. In this study, MA-10 cells were treated with different reagents in the presence or absence of CS (10 mg/ml) for 3 h to determine the mechanisms. Results illustrated that CS activated the Gsalpha protein subunit, but not Gialpha, to induce cell steroidogenesis. Moreover, PKA inhibitors inhibited 37% of CS-stimulated steroidogenesis, which demonstrated that CS might enhance the cAMP-PKA pathway to affect MA-10 cell steroidogenesis. Because of incomplete inhibition by PKA inhibitors, we also examined the PKC pathway. PKC inhibitor, phospholipase C inhibitor, and calmodulin antagonist blocked 35-52% of CS-stimulated steroidogenesis in MA-10 cells, strongly suggesting that CS had activated the PKC pathway. Co-treatment with PKA and PKC inhibitors abolished 61% of CS-stimulated steroid production, indicating that CS simultaneously activated PKA and PKC pathways. Moreover, CS induced the expression of steroidogenic acute regulatory (StAR) protein in dose- and time-dependent relationships, and PKA inhibitor, PKC inhibitor, or co-treatment with both inhibitors suppressed it. These data support that CS activat...Continue Reading

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Citations

Oct 6, 2010·Journal of Industrial Microbiology & Biotechnology·C-H Dong, Y-J Yao
Jan 10, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Chun-Yi JenSew-Fen Leu
Apr 2, 2013·Chinese Journal of Integrative Medicine·Ying-hui ChenBu-miin Huang
Jul 1, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Bo-Syong PanBu-Miin Huang
Apr 5, 2016·Pharmacognosy Reviews·Kanitta Jiraungkoorskul, Wannee Jiraungkoorskul
Oct 28, 2009·Toxicology Letters·Edmund Cheung SoBu-Miin Huang
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Sep 24, 2004·The International Journal of Biochemistry & Cell Biology·Yung-Chia ChenBu-Miin Huang
Oct 30, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei-Nan WangZhi-Dong Qiu
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Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Ying ChenRong-Jane Chen

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