Core cis-element variation confers subgenome-biased expression of a transcription factor that functions in cotton fiber elongation

The New Phytologist
Bo ZhaoXiao-Ya Chen

Abstract

Cotton cultivars have evolved to produce extensive, long, seed-born fibers important for the textile industry, but we know little about the molecular mechanism underlying spinnable fiber formation. Here, we report how PACLOBUTRAZOL RESISTANCE 1 (PRE1) in cotton, which encodes a basic helix-loop-helix (bHLH) transcription factor, is a target gene of spinnable fiber evolution. Differential expression of homoeologous genes in polyploids is thought to be important to plant adaptation and novel phenotypes. PRE1 expression is specific to cotton fiber cells, upregulated during their rapid elongation stage and A-homoeologous biased in allotetraploid cultivars. Transgenic studies demonstrated that PRE1 is a positive regulator of fiber elongation. We determined that the natural variation of the canonical TATA-box, a regulatory element commonly found in many eukaryotic core promoters, is necessary for subgenome-biased PRE1 expression, representing a mechanism underlying the selection of homoeologous genes. Thus, variations in the promoter of the cell elongation regulator gene PRE1 have contributed to spinnable fiber formation in cotton. Overexpression of GhPRE1 in transgenic cotton yields longer fibers with improved quality parameters, in...Continue Reading

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Citations

Jun 29, 2018·The Plant Journal : for Cell and Molecular Biology·Verandra KumarSamir V Sawant
Jan 26, 2020·Trends in Plant Science·Zuoren YangFuguang Li
Jan 12, 2021·Annual Review of Plant Biology·Gai HuangYu-Xian Zhu

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Methods Mentioned

BETA
transgenic
scraping
reverse transcription PCR
PCR
Assay
genotyping

Software Mentioned

BLAST
ega
MEGA

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