Core formation in apomyoglobin: probing the upper reaches of the folding energy landscape

Biochemistry
M GulottaR B Dyer

Abstract

An acid-destabilized form of apomyoglobin, the so-called E state, consists of a set of heterogeneous structures that are all characterized by a stable hydrophobic core composed of 30-40 residues at the intersection of the A, G, and H helices of the protein, with little other secondary structure and no other tertiary structure. Relaxation kinetics studies were carried out to characterize the dynamics of core melting and formation in this protein. The unfolding and/or refolding response is induced by a laser-induced temperature jump between the folded and unfolded forms of E, and structural changes are monitored using the infrared amide I' absorbance at 1648-1651 cm(-1) that reports on the formation of solvent-protected, native-like helix in the core and by fluorescence emission changes from apomyoglobin's Trp14, a measure of burial of the indole group of this residue. The fluorescence kinetics data are monoexponential with a relaxation time of 14 micros. However, infrared kinetics data are best fit to a biexponential function with relaxation times of 14 and 59 micros. These relaxation times are very fast, close to the limits placed on folding reactions by diffusion. The 14 micros relaxation time is weakly temperature dependent a...Continue Reading

References

Feb 1, 1997·Current Opinion in Structural Biology·W A EatonJ Hofrichter
Mar 26, 1998·Journal of Molecular Biology·M Jamin, R L Baldwin

❮ Previous
Next ❯

Citations

Sep 13, 2002·Journal of Molecular Biology·Chiaki NishimuraPeter E Wright
Sep 25, 2002·Journal of Molecular Biology·Michael A LietzowPeter E Wright
Jan 12, 2002·Current Opinion in Structural Biology·M T Zanni, R M Hochstrasser
Feb 13, 2003·Current Opinion in Structural Biology·Neil Ferguson, Alan R Fersht
Dec 5, 2002·Current Opinion in Structural Biology·Robert Callender, R Brian Dyer
May 11, 2006·Chemical Reviews·Heinrich RoderHong Cheng
Jun 5, 2002·Annual Review of Biochemistry·Jeffrey K Myers, Terrence G Oas
Feb 8, 2005·Applied Bioinformatics·Christopher BystroffXin Yuan
Jan 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takanori UzawaTetsuro Fujisawa
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Heinrich Roder
Aug 16, 2005·Biopolymers·Nicolas TaulierTigran V Chalikian
Jul 9, 2004·Journal of Molecular Biology·Ronaldo Mohana-BorgesPeter E Wright
Jul 22, 2009·Biophysical Journal·Martin LignellHans-Christian Becker
Mar 1, 2003·Biophysical Journal·Miriam GulottaR Brian Dyer
Dec 30, 2016·Accounts of Chemical Research·H Jane Dyson, Peter E Wright
Aug 12, 2004·Chemical Reviews·H Jane Dyson, Peter E Wright
Jul 14, 2018·The Journal of Physical Chemistry. B·Angela E VarelaSilvia Cavagnero
Jul 21, 2006·The Journal of Physical Chemistry. B·Aleksandr V Mikhonin, Sanford A Asher
May 27, 2004·Journal of the American Chemical Society·Dung M VuR Brian Dyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.