Core-labelling technique (CLT): a novel combination of the ingrowth-core method and tracer technique for deep root study

Plant Methods
Eusun HanKristian Thorup-Kristensen

Abstract

Ingrowth-core method is a useful tool to determine fine root growth of standing crops by inserting root-free soil in mesh-bags for certain period of time. However, the root density observed by the method does not directly explain the nutrient uptake potential of crop plants as it varies over soil depth and incubation time. We have inserted an access-tube up to 4.2 m of soil depth with openings directly under crop plants, through which ingrowth-cores containing labelled soil with nutrient tracers were installed, called core-labelling technique (CLT). The main advantage of CLT would be its capacity to determine both root density and root activity from the same crop plants in deep soil layers. We tested the validity of the new method using a model crop species, alfalfa (Medicago sativa) against three depth-levels (1.0, 2.5 and 4.2 m), three sampling spots with varying distance (0-0.36, 0.36-0.72 and > 5 m from core-labelled spot), two sampling times (week 4 and 8), and two plant parts (young and old leaves) under two field experiments (spring and autumn). Using CLT, we were able to observe both deep root growth and root activity up to 4.2 m of soil depth. Tracer concentrations revealed that there was no sign of tracer-leakage to a...Continue Reading

References

Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·N. TerryA. S. Tarun
Oct 1, 1940·Plant Physiology·J D Sayre, V H Morris
Oct 1, 1941·Plant Physiology·R Collander
Nov 10, 2009·Plant, Cell & Environment·Rosemary G White, John A Kirkegaard
Jan 15, 2015·Journal of Experimental Botany·Jonathan P Lynch, Tobias Wojciechowski
Jun 6, 2016·Plant Physiology and Biochemistry : PPB·Babar ShahzadIftikhar Ali
Oct 4, 2016·Plant Signaling & Behavior·Zsuzsanna KolbertGábor Feigl
Dec 4, 2019·Trends in Plant Science·Anton P WassonMichelle Watt

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Software Mentioned

lme4
R Core
WinRHIZO Pro
CLT
multcomp
lmerTest
R

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