Core mutants of the immunoglobulin binding domain of streptococcal protein G: stability and structural integrity

FEBS Letters
Angela M GronenbornG M Clore

Abstract

A library of core mutants of the GB1 domain of streptococcal protein G was created, and the structure and stability of selected members was assessed by 1H-15N heteronuclear correlation NMR spectroscopy and fluorescence. All mutants comprised changes in beta-sheet residues, with sidechains at positions 5 (Leu), 7 (Leu), 52 (Phe) and 54 (Val) forming the beta-sheet side of the sheet-helix core interface. A solvent exposed position Ile-6 was chosen as a control. Randomization of bases at codon positions 1 and 3 with thymine at position 2 introduces five possible hydrophobic amino acids, namely Leu, Val, Ile, Phe, and Met. The distribution of encoded amino acids at all five positions is approximately as expected theoretically and indicates that no major bias was introduced towards particular residues. The overall structural integrity of several mutants, as assessed by NMR, ranges from very close to wild type to fully unfolded. Interestingly, the stability of the mutants is not strictly correlated with the number of changes or residue volume.

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Mar 20, 1991·Journal of Molecular Biology·L SerranoA R Fersht
Nov 1, 1994·Protein Science : a Publication of the Protein Society·M MunsonL Regan
Oct 1, 1995·Nature Structural Biology·A R DavidsonR T Sauer
Sep 1, 1994·Nature Structural Biology·F J BlancoL Serrano
May 1, 1995·Nature Structural Biology·M KlembaL Regan
Nov 1, 1994·Protein Science : a Publication of the Protein Society·J KuszewskiA M Gronenborn
Nov 1, 1993·Quarterly Reviews of Biophysics·F M Richards, W A Lim
Sep 15, 1994·Nature·D L Minor, P S Kim
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·A R Davidson, R T Sauer
Jan 1, 1993·Annual Review of Biochemistry·B W Matthews
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·D D AxeA R Fersht
Jan 1, 1996·Protein Science : a Publication of the Protein Society·A M Gronenborn, G M Clore

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Citations

Jan 20, 2004·Journal of Molecular Biology·Keyang DingAngela M Gronenborn
Oct 1, 2003·Journal of Molecular Biology·In-Ja L ByeonAngela M Gronenborn
Aug 1, 1997·Current Opinion in Structural Biology·P Kast, D Hilvert
Sep 1, 1999·Chemistry & Biology·S F Marino, L Regan
Jan 13, 2000·Protein Science : a Publication of the Protein Society·B OdaertG Lippens
Apr 21, 2004·European Journal of Biochemistry·Thomas J Magliery, Lynne Regan
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·William B Monteith, Gary J Pielak
Nov 5, 1999·Journal of Molecular Biology·P Koehl, M Levitt
Feb 18, 2016·FEBS Letters·Jacopo MarinoRoland Beckmann
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Amita Datta, Martin J Stone
Dec 14, 2007·Proteins·JunGoo JeeAngela M Gronenborn
Sep 16, 2009·Biophysical Journal·Enrico GuarneraAmedeo Caflisch
Mar 27, 2018·Protein Science : a Publication of the Protein Society·Connie Y WangBarry D Olafson
Apr 23, 2008·Current Protocols in Protein Science·Angela M Gronenborn
Jul 8, 2016·Chembiochem : a European Journal of Chemical Biology·Eric BlockHuawei Zeng
Sep 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·José Arcadio Farías-RicoGunnar von Heijne
Dec 18, 2019·The Journal of Physical Chemistry. B·Qingqing ChenSharon Rozovsky

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